BRENDA - Enzyme Database show

Purification of deoxycytidine kinases from two P815 murine neoplasms and their separation from deoxyguanosine kinase

Meyers, M.B.; Kreis, W.; Arch. Biochem. Biophys. 177, 10-15 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
activation
Mus musculus
General Stability
EC Number
General Stability
Organism
2.7.1.74
dithiothreitol stabilizes
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
37°C, pH 8.0
Mus musculus
2.7.1.74
0.014
0.0167
deoxycytidine
37°C, pH 8.0; cytosine arabinoside-induced cells, 37°C, pH 8.0
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
carcinoma cell line
P815-neoplasm cell line
Mus musculus
-
2.7.1.74
P-815 cell
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00088
-
cytosine arabinoside-induced cells
Mus musculus
2.7.1.74
0.0846
-
37°C, pH 8.0
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641933
Mus musculus
ADP + 5'-dCMP
-
641933
Mus musculus
?
2.7.1.74
NTP + deoxycytidine
no substrate: deoxyuridine
641933
Mus musculus
NDP + dCMP
-
641933
Mus musculus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Mus musculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
1330
-
deoxycytidine
cytosine arabinoside-induced cells
Mus musculus
2.7.1.74
78300
-
deoxycytidine
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
activation
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.74
dithiothreitol stabilizes
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
37°C, pH 8.0
Mus musculus
2.7.1.74
0.014
0.0167
deoxycytidine
37°C, pH 8.0; cytosine arabinoside-induced cells, 37°C, pH 8.0
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
carcinoma cell line
P815-neoplasm cell line
Mus musculus
-
2.7.1.74
P-815 cell
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00088
-
cytosine arabinoside-induced cells
Mus musculus
2.7.1.74
0.0846
-
37°C, pH 8.0
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641933
Mus musculus
ADP + 5'-dCMP
-
641933
Mus musculus
?
2.7.1.74
NTP + deoxycytidine
no substrate: deoxyuridine
641933
Mus musculus
NDP + dCMP
-
641933
Mus musculus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Mus musculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
1330
-
deoxycytidine
cytosine arabinoside-induced cells
Mus musculus
2.7.1.74
78300
-
deoxycytidine
-
Mus musculus