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Literature summary extracted from

  • Momparler, R.L.; Fischer, G.A.
    Mammalian deoxynucleoside kinase. I. Deoxycytidine kinase: purification, properties, and kinetic studies with cytosine arabinoside (1968), J. Biol. Chem., 243, 4298-4304.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.74 dithiothreitol not Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 cytosine arabinoside deoxycytidine as substrate Bos taurus
2.7.1.74 dCDP
-
 Bos taurus
2.7.1.74 dCMP
-
 Bos taurus
2.7.1.74 dCTP
-
 Bos taurus
2.7.1.74 deoxyadenosine not Bos taurus
2.7.1.74 deoxycytidine cytosine arabinoside as substrate Bos taurus
2.7.1.74 deoxyguanosine not Bos taurus
2.7.1.74 HgCl2
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.014
-
 deoxycytidine 37°C, pH 8.0 Bos taurus
2.7.1.74 0.014 0.0167 deoxycytidine 37°C, pH 8.0 Bos taurus
2.7.1.74 0.02 0.04 cytosine arabinoside 37°C, pH 8.0 Bos taurus
2.7.1.74 0.11
-
 ATP cosubstrate deoxycytidine, 37°C, pH 8.0 Bos taurus
2.7.1.74 0.2
-
 ATP cosubstrate cytosine arabinoside, 37°C, pH 8.0 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Bos taurus
2.7.1.74 Ca2+ can replace Mn2+ or Mg2+ to some extent Bos taurus
2.7.1.74 Mg2+ more efficient than Mn2+ or Ca2+ Bos taurus
2.7.1.74 Mn2+ requirement Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.00632
-
 37°C, pH 8.0 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Bos taurus ADP + 5'-dCMP
-
 ?
2.7.1.74 CTP + 2'-deoxycytidine
-
 Bos taurus CDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dGTP + 2'-deoxycytidine
-
 Bos taurus dGDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dTTP + 2'-deoxycytidine
-
 Bos taurus dTDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 GTP + 2'-deoxycytidine best phosphate donor Bos taurus GDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 NTP + cytosine arabinoside ATP can be replaced by GTP, dATP, dGTP or dTTP Bos taurus NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxycytidine enzyme shows little specificity towards phosphate donor Bos taurus NDP + dCMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Bos taurus