BRENDA - Enzyme Database show

Mammalian deoxynucleoside kinase. I. Deoxycytidine kinase: purification, properties, and kinetic studies with cytosine arabinoside

Momparler, R.L.; Fischer, G.A.; J. Biol. Chem. 243, 4298-4304 (1968)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
not
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Bos taurus
2.7.1.74
dCDP
-
Bos taurus
2.7.1.74
dCMP
-
Bos taurus
2.7.1.74
dCTP
-
Bos taurus
2.7.1.74
deoxyadenosine
not
Bos taurus
2.7.1.74
deoxycytidine
cytosine arabinoside as substrate
Bos taurus
2.7.1.74
deoxyguanosine
not
Bos taurus
2.7.1.74
HgCl2
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.014
0.0167
deoxycytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
0.014
-
deoxycytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
0.02
0.04
cytosine arabinoside
37°C, pH 8.0
Bos taurus
2.7.1.74
0.11
-
ATP
cosubstrate deoxycytidine, 37°C, pH 8.0
Bos taurus
2.7.1.74
0.2
-
ATP
cosubstrate cytosine arabinoside, 37°C, pH 8.0
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation; can replace Mn2+ or Mg2+ to some extent
Bos taurus
2.7.1.74
Mg2+
more efficient than Mn2+ or Ca2+
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
partial
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00632
-
37°C, pH 8.0
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641930
Bos taurus
ADP + 5'-dCMP
-
641930
Bos taurus
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641930
Bos taurus
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
-
641930
Bos taurus
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641930
Bos taurus
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
best phosphate donor
641930
Bos taurus
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
ATP can be replaced by GTP, dATP, dGTP or dTTP
641930
Bos taurus
NDP + cytosine arabinoside 5'-monophosphate
-
641930
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641930
Bos taurus
NDP + dCMP
-
641930
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
not
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Bos taurus
2.7.1.74
dCDP
-
Bos taurus
2.7.1.74
dCMP
-
Bos taurus
2.7.1.74
dCTP
-
Bos taurus
2.7.1.74
deoxyadenosine
not
Bos taurus
2.7.1.74
deoxycytidine
cytosine arabinoside as substrate
Bos taurus
2.7.1.74
deoxyguanosine
not
Bos taurus
2.7.1.74
HgCl2
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.014
0.0167
deoxycytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
0.014
-
deoxycytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
0.02
0.04
cytosine arabinoside
37°C, pH 8.0
Bos taurus
2.7.1.74
0.11
-
ATP
cosubstrate deoxycytidine, 37°C, pH 8.0
Bos taurus
2.7.1.74
0.2
-
ATP
cosubstrate cytosine arabinoside, 37°C, pH 8.0
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation; can replace Mn2+ or Mg2+ to some extent
Bos taurus
2.7.1.74
Mg2+
more efficient than Mn2+ or Ca2+
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
partial
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00632
-
37°C, pH 8.0
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641930
Bos taurus
ADP + 5'-dCMP
-
641930
Bos taurus
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641930
Bos taurus
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
-
641930
Bos taurus
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641930
Bos taurus
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
best phosphate donor
641930
Bos taurus
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
ATP can be replaced by GTP, dATP, dGTP or dTTP
641930
Bos taurus
NDP + cytosine arabinoside 5'-monophosphate
-
641930
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641930
Bos taurus
NDP + dCMP
-
641930
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus