EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.71 | 0.16 | - |
ATP | isoenzyme SK2, at 1 mM shikimate | Escherichia coli | |
2.7.1.71 | 0.2 | - |
shikimate | isoenzyme SK2, at 5 mM ATP | Escherichia coli | |
2.7.1.71 | 5 | - |
shikimate | above, isoenzyme SK1, at 5 mM | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.71 | Ca2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
2.7.1.71 | Co2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
2.7.1.71 | Fe2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
2.7.1.71 | Mg2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
2.7.1.71 | Mn2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.71 | 19000 | - |
isoenzyme SK1, gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.71 | ATP + shikimate | Escherichia coli | SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs | ADP + shikimate 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.71 | Escherichia coli | - |
- |
- |
2.7.1.71 | Escherichia coli | - |
two isoenzymes: SK1 and SK2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.71 | isoenzyme SK1 and SK2 | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.71 | 100 | - |
isoenzyme SK2 | Escherichia coli |
2.7.1.71 | 410 | - |
isoenzyme SK1 | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.71 | ATP + shikimate | - |
Escherichia coli | ADP + shikimate 3-phosphate | - |
? | |
2.7.1.71 | ATP + shikimate | SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs | Escherichia coli | ADP + shikimate 3-phosphate | - |
? |