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Literature summary extracted from

  • Kwok, F.; Humpage, A.; Cheng, C.H.K.
    Phosphatidylinositol phosphate 5-kinase: purification and inhibition studies (1996), Prep. Biochem. Biotechnol., 26, 1-19.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.68 2'(3')-O-(2,4,6-Trinitrophenyl)ATP competitive Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 0.043
-
 ATP pH 7.4, 30°C Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.68 membrane
-
 Ovis aries 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.68 60000
-
 x * 60000, SDS-PAGE Ovis aries
2.7.1.68 130000
-
 gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 brain
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 1.75
-
-
 Ovis aries

Storage Stability

EC Number Storage Stability Organism
2.7.1.68 4°C, about 10% loss of activity after overnight storage Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Ovis aries ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate no activity Ovis aries GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.68 dimer x * 60000, SDS-PAGE Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.68 0.055
-
 2'(3')-O-(2,4,6-Trinitrophenyl)ATP pH 7.4, 30°C Ovis aries