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Literature summary extracted from

  • Bazenet, C.E.; Anderson, R.A.
    Phosphatidylinositol-4-phosphate 5-kinases from human erythrocytes (1992), Methods Enzymol., 209, 189-202.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.68 heparin type I enzyme is stimulated at low concentrations Homo sapiens
2.7.1.68 spermine 2 mM, 4fold increase in activity of type I enzyme, no effect on type II enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.68 heparin type II kinase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 0.0012
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate in 1-phosphatidyl-1D-myo-inositol 4-phosphate in membranes, type I enzyme Homo sapiens
2.7.1.68 0.0014
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate in 1-phosphatidyl-1D-myo-inositol 4-phosphate liposomes, type I enzyme Homo sapiens
2.7.1.68 0.005
-
 ATP type II enzyme Homo sapiens
2.7.1.68 0.006
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate in 1-phosphatidyl-1D-myo-inositol 4-phosphate micelles, type I enzyme Homo sapiens
2.7.1.68 0.025
-
 ATP type I enzyme Homo sapiens
2.7.1.68 0.04
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate in 1-phosphatidyl-1D-myo-inositol 4-phosphate liposomes, type II enzyme Homo sapiens
2.7.1.68 0.06
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate in 1-phosphatidyl-1D-myo-inositol 4-phosphate micelles, type II enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.68 cytosol type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic Homo sapiens 5829
-
2.7.1.68 membrane type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.68 53000
-
 x * 53000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68 cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 erythrocyte two form of PIP kinase: type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 0.015
-
 membrane bound type II enzyme Homo sapiens
2.7.1.68 0.023
-
 cytosolic enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate 0.05 mM GTP is 2fold more active than ATP with type II enzyme. 0.05 mM GTP is 5fold more active than ATP with the type I enzyme Homo sapiens GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.68 ? x * 53000, SDS-PAGE Homo sapiens