Literature summary extracted from

Zhao, X.H.; Bondeva, T.; Balla, T.
Characterization of recombinant phosphatidylinositol 4-kinase beta reveals auto- and heterophosphorylation of the enzyme (2000), J. Biol. Chem., 275, 14642-14648.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.1.67	glutathione S-transferase PI4Kbeta fusion protein, expression in Escherichia coli	Bos taurus

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.7.1.67	additional information	creation of a glutathione S-transferase PI4Kbeta fusion protein that is biologically active and phosphorylates phosphatidylinositol in ist 4-position with wortmannin sensitivity and kinetic parameters that are identical to those of purified wild-type enzyme	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.67	Bos taurus	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
2.7.1.67	phosphoprotein	enzyme exhibits autophosphorylation that is enhanced by Mn2+ ions. Isolated C-terminal catalytic domain still displays autophosphorylation. Phosphorylation of endogenous or overexpressed PI4Kbeta is also observed in COS-7 cells	Bos taurus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.67	glutathione S-transferase PI4Kbeta fusion protein	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.1.67	brain	-	Bos taurus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.67	ATP + 1-phosphatidyl-1D-myo-inositol	-	Bos taurus	ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	?

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Release 2023.1 (January 2023)