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Literature summary extracted from

  • Husebye, E.S.; Letcher, A.J.; Lander, D.J.; Flatmark, T.
    Purification and kinetic properties of a membrane-bound phosphatidylinositol kinase of the bovine adrenal medulla (1990), Biochim. Biophys. Acta, 1042, 330-337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 poly(L-lysine) stimulates Bos taurus
2.7.1.67 Trifluoperazine stimulates activity of the purified enzyme less efficiently than that of the membrane-bound enzyme Bos taurus
2.7.1.67 Triton X-100 optimal activity at about 0.1% w/v Bos taurus
2.7.1.67 Triton X-100 maximal stimulation at 0.1% w/v Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine IC50: 0.07 mM Bos taurus
2.7.1.67 Ca2+
-
 Bos taurus
2.7.1.67 MES IC50: 4 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.017
-
 phosphatidylinositol pH 7.0, 25°C Bos taurus
2.7.1.67 0.04
-
 phosphatidylinositol
-
 Bos taurus
2.7.1.67 0.055
-
 ATP pH 7.0, 30°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 chromaffin granule
-
 Bos taurus 42583
-
2.7.1.67 membrane integral membrane protein Bos taurus 16020
-
2.7.1.67 membrane bound to Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ stimulates Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 adrenal medulla
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 0.0051
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.67 7
-
 broad, around Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.67 0.07
-
 IC50: 0.07 mM Bos taurus adenosine
2.7.1.67 4
-
 IC50: 4 mM Bos taurus MES