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Literature summary extracted from

  • McKenzie, M.A.; Carman, G.M.
    Membrane-associated phosphatidylinositol kinase from Saccharomyces cerevisiae (1983), J. Bacteriol., 156, 421-423.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 sodium cholate stimulates 3.8fold at 14 mM Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 F-
-
 Saccharomyces cerevisiae
2.7.1.67 K+
-
 Saccharomyces cerevisiae
2.7.1.67 Li+
-
 Saccharomyces cerevisiae
2.7.1.67 Na+
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.071
-
 phosphatidylinositol pH 8.5, 30°C Saccharomyces cerevisiae
2.7.1.67 0.21
-
 ATP pH 8.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 membrane associated Saccharomyces cerevisiae 16020
-
2.7.1.67 microsome
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ dependent on, maximal activity at 10 mM Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Saccharomyces cerevisiae
-
 S288c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 0.0933
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.67 -80°C, about 50% loss of activity after 2 weeks Saccharomyces cerevisiae
2.7.1.67 8°C, 30% loss of activity after 4 h Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Saccharomyces cerevisiae ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.67 8.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.67 6 9 active from pH 6.0 to pH 9.0 with Tris maleate buffer Saccharomyces cerevisiae