Literature summary extracted from

Yang, J.; Fu, X.; Jia, Q.; Shen, J.; Biggins, J.B.; Jiang, J.; Zhao, J.; Schmidt, J.J.; Wang, P.G.; Thorson, J.S.
Studies on the substrate specificity of Escherichia coli galactokinase (2003), Org. Lett., 5, 2223-2226.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.1.6	gene from Escherichia coli K-12 is subcloned into pET-15b vector and overexpressed in BL21 cells	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.6	2.1	-	D-galactose	-	Escherichia coli
2.7.1.6	2.5	-	ATP	-	Escherichia coli
2.7.1.6	2.9	-	2-amino-deoxy-D-galactose	-	Escherichia coli
2.7.1.6	3.6	-	2-deoxy-D-galactose	-	Escherichia coli
2.7.1.6	4.9	-	6-deoxy-D-galactose	-	Escherichia coli
2.7.1.6	6.4	-	3-deoxy-3--methyl-D-galactose	-	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.6	Escherichia coli	-	K-12	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.6	ATP + 2-amino-2-deoxy-D-galactose	-	Escherichia coli	ADP + 2-amino-2-deoxy-D-galactose 1-phosphate	-	?
2.7.1.6	ATP + 2-deoxy-D-galactose	-	Escherichia coli	ADP + 2-deoxy-D-galactose 1-phosphate	-	?
2.7.1.6	ATP + 3-deoxy-3-methyl-D-galactose	-	Escherichia coli	ADP + 3-deoxy-3-methyl-D-galactose 1-phosphate	-	?
2.7.1.6	ATP + 6-deoxy-D-galactose	-	Escherichia coli	ADP + 6-deoxy-D-galactose 1-phosphate	-	?
2.7.1.6	ATP + D-galactose	-	Escherichia coli	ADP + alpha-D-galactose 1-phosphate	-	?
2.7.1.6	additional information	no tolerance for C-4 changes in the substrate	Escherichia coli	?	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.7.1.6	0.0483	-	6-deoxy-D-galactose	-	Escherichia coli
2.7.1.6	0.085	-	3-deoxy-3-methyl-D-galactose	-	Escherichia coli
2.7.1.6	0.195	-	2-amino-deoxy-D-galactose	-	Escherichia coli
2.7.1.6	0.5	-	2-deoxy-D-galactose	-	Escherichia coli

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Release 2023.1 (January 2023)