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Literature summary extracted from

  • Srivastava, S.K.; Blume, K.G.; Van Loon, C.; Beutler, E.
    Purification and kinetic properties of galactokinase from human placenta (1972), Arch. Biochem. Biophys., 150, 191-198.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.6 dithiothreitol protects the enzyme better than 2-mercaptoethanol against denaturation on storage at 4°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 alpha-D-galactose 1-phosphate noncompetitive with respect to galactose Homo sapiens
2.7.1.6 D-galactose 1-phosphate
-
 Homo sapiens
2.7.1.6 Hg2+
-
 Homo sapiens
2.7.1.6 NEM 1.0 mM, 41% inhibition Homo sapiens
2.7.1.6 PCMB 1.0 mM, complete inhibition Homo sapiens
2.7.1.6 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.1
-
 ATP 37°C Homo sapiens
2.7.1.6 0.25
-
 D-galactose 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Ca2+ absolute requirement for a divalent cation, Ca2+ can be partially replaced by Co2+ Homo sapiens
2.7.1.6 Co2+ absolute requirement for a divalent cation, Mg2+ can be partially replaced by Co2+ Homo sapiens
2.7.1.6 Mg2+ absolute requirement for a divalent cation, maximally active in presence of 5 mM Mg2+ Homo sapiens
2.7.1.6 Mn2+ absolute requirement for a divalent cation, Mg2+ can be partially replaced by Co2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 58000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.6 erythrocyte
-
 Homo sapiens
-
2.7.1.6 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.6 0.15
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + D-galactose
-
 Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.6 30
-
 10 min, no loss of activity Homo sapiens
2.7.1.6 50
-
 10 min, 25% loss of activity Homo sapiens
2.7.1.6 60
-
 10 min, 60% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 7.6
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.6 2
-
 D-galactose 1-phosphate 37°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.6 Homo sapiens isoelectric focusing
-
 5.8