BRENDA - Enzyme Database

Purification and characterization of cytosolic pyruvate kinase from developing seeds of Brassica campestris L

Singh, D.K.; Malhotra, S.P.; Singh, R.; Indian J. Biochem. Biophys. 37, 51-58 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
-
Brassica rapa subsp. oleifera
2.7.1.40
D-glucose 6-phosphate
-
Brassica rapa subsp. oleifera
2.7.1.40
phosphate
-
Brassica rapa subsp. oleifera
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
citrate
-
Brassica rapa subsp. oleifera
2.7.1.40
fumarate
-
Brassica rapa subsp. oleifera
2.7.1.40
glutamine
-
Brassica rapa subsp. oleifera
2.7.1.40
isocitrate
-
Brassica rapa subsp. oleifera
2.7.1.40
L-glutamate
-
Brassica rapa subsp. oleifera
2.7.1.40
oxalate
-
Brassica rapa subsp. oleifera
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.1
-
phosphoenolpyruvate
pH 6.8, 30°C
Brassica rapa subsp. oleifera
2.7.1.40
0.11
-
ADP
pH 6.8, 30°C
Brassica rapa subsp. oleifera
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
cytosol
-
Brassica rapa subsp. oleifera
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
fulfills requirement for monovalent cation
Brassica rapa subsp. oleifera
2.7.1.40
Mg2+
fulfills absolute requirement for divalent cation
Brassica rapa subsp. oleifera
2.7.1.40
additional information
absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this
Brassica rapa subsp. oleifera
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
55000
-
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
2.7.1.40
57000
-
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
2.7.1.40
214000
-
gel filtration
Brassica rapa subsp. oleifera
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Brassica rapa subsp. oleifera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
-
Brassica rapa subsp. oleifera
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.40
ATP + pyruvate = ADP + phosphoenolpyruvate
compulsory-ordered tri-bi mechanism
Brassica rapa subsp. oleifera
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
seed
-
Brassica rapa subsp. oleifera
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
poor substrates: UDP, GDP
641612
Brassica rapa subsp. oleifera
ATP + pyruvate
-
-
-
?
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641612
Brassica rapa subsp. oleifera
ATP + pyruvate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.8
-
-
Brassica rapa subsp. oleifera
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
-
Brassica rapa subsp. oleifera
2.7.1.40
D-glucose 6-phosphate
-
Brassica rapa subsp. oleifera
2.7.1.40
phosphate
-
Brassica rapa subsp. oleifera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
citrate
-
Brassica rapa subsp. oleifera
2.7.1.40
fumarate
-
Brassica rapa subsp. oleifera
2.7.1.40
glutamine
-
Brassica rapa subsp. oleifera
2.7.1.40
isocitrate
-
Brassica rapa subsp. oleifera
2.7.1.40
L-glutamate
-
Brassica rapa subsp. oleifera
2.7.1.40
oxalate
-
Brassica rapa subsp. oleifera
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.1
-
phosphoenolpyruvate
pH 6.8, 30°C
Brassica rapa subsp. oleifera
2.7.1.40
0.11
-
ADP
pH 6.8, 30°C
Brassica rapa subsp. oleifera
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
cytosol
-
Brassica rapa subsp. oleifera
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
fulfills requirement for monovalent cation
Brassica rapa subsp. oleifera
2.7.1.40
Mg2+
fulfills absolute requirement for divalent cation
Brassica rapa subsp. oleifera
2.7.1.40
additional information
absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this
Brassica rapa subsp. oleifera
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
55000
-
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
2.7.1.40
57000
-
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
2.7.1.40
214000
-
gel filtration
Brassica rapa subsp. oleifera
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
-
Brassica rapa subsp. oleifera
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
seed
-
Brassica rapa subsp. oleifera
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
poor substrates: UDP, GDP
641612
Brassica rapa subsp. oleifera
ATP + pyruvate
-
-
-
?
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641612
Brassica rapa subsp. oleifera
ATP + pyruvate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
x * 55000 + 4-x * 57000, SDS-PAGE
Brassica rapa subsp. oleifera
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.8
-
-
Brassica rapa subsp. oleifera