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Literature summary extracted from

  • Abe, K.; Yamada, T.
    Purification and properties of pyruvate kinase from Streptococcus mutans (1982), J. Bacteriol., 149, 299-305.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate not Streptococcus mutans
2.7.1.40 D-glucose 6-phosphate requirement Streptococcus mutans
2.7.1.40 D-ribose 5-phosphate activation Streptococcus mutans
2.7.1.40 D-Ribulose 1,5-diphosphate not Streptococcus mutans
2.7.1.40 glyceraldehyde 3-phosphate not Streptococcus mutans
2.7.1.40 additional information no activation by glucose 1,6-diphosphate, 5'-IMP, 2',3'-AMP, 2',3'-GMP, 2',3'-UMP Streptococcus mutans

General Stability

EC Number General Stability Organism
2.7.1.40 dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7 Streptococcus mutans
2.7.1.40 phosphate restores activity of dialyzed, partially purified, not fully purified, enzyme preparations Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 phosphate at high concentrations; strong Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
additional information kinetic parameters Streptococcus mutans
2.7.1.40 0.026
-
GDP pH 7.5 Streptococcus mutans
2.7.1.40 0.04 0.063 phosphoenolpyruvate pH 7.5 Streptococcus mutans
2.7.1.40 0.049 0.1 IDP
-
Streptococcus mutans
2.7.1.40 0.22
-
phosphoenolpyruvate in the presence of glucose 6-phosphate, at pH 7.5 Streptococcus mutans
2.7.1.40 0.25 0.26 GDP UDP, at pH 7.5 Streptococcus mutans
2.7.1.40 0.39
-
ADP in the presence of glucose 6-phosphate, pH 7.5 Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Ca2+ not Streptococcus mutans
2.7.1.40 divalent cation requirement Streptococcus mutans
2.7.1.40 divalent cation requires both a divalent and a monovalent cation Streptococcus mutans
2.7.1.40 K+ requirement Streptococcus mutans
2.7.1.40 K+ only together with activating divalent cation Streptococcus mutans
2.7.1.40 Mg2+ requirement Streptococcus mutans
2.7.1.40 Mn2+ requirement Streptococcus mutans
2.7.1.40 Mn2+ only together with K+ Streptococcus mutans
2.7.1.40 monovalent cation requirement Streptococcus mutans
2.7.1.40 monovalent cation requires both a divalent and a monovalent cation Streptococcus mutans
2.7.1.40 additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Streptococcus mutans
2.7.1.40 additional information no activation by Li+ Streptococcus mutans
2.7.1.40 Na+ not Streptococcus mutans
2.7.1.40 NH4+ requirement Streptococcus mutans
2.7.1.40 NH4+ can replace K+ Streptococcus mutans
2.7.1.40 NH4+ only together with activating divalent cation Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 44000
-
4 * 44000, SDS-PAGE Streptococcus mutans
2.7.1.40 180000 190000 gel filtration Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Streptococcus mutans
-
-
-
2.7.1.40 Streptococcus mutans JC2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, in 40 mM potassium phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 3 months Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
Streptococcus mutans ATP + pyruvate
-
?
2.7.1.40 ADP + phosphoenolpyruvate
-
Streptococcus mutans JC2 ATP + pyruvate
-
?
2.7.1.40 CDP + phosphoenolpyruvate poor substrate Streptococcus mutans CTP + pyruvate
-
?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans CTP + pyruvate
-
?
2.7.1.40 CDP + phosphoenolpyruvate poor substrate Streptococcus mutans JC2 CTP + pyruvate
-
?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 CTP + pyruvate
-
?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans GTP + pyruvate
-
?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 GTP + pyruvate
-
?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans ITP + pyruvate
-
?
2.7.1.40 TDP + phosphoenolpyruvate poor substrate Streptococcus mutans TTP + pyruvate
-
?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans UTP + pyruvate
-
?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 UTP + pyruvate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 44000, SDS-PAGE Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8 7.7
-
Streptococcus mutans

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP not Streptococcus mutans
2.7.1.40 GMP not Streptococcus mutans