EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | D-fructose 1,6-diphosphate | not | Streptococcus mutans | |
2.7.1.40 | D-glucose 6-phosphate | requirement | Streptococcus mutans | |
2.7.1.40 | D-ribose 5-phosphate | activation | Streptococcus mutans | |
2.7.1.40 | D-Ribulose 1,5-diphosphate | not | Streptococcus mutans | |
2.7.1.40 | glyceraldehyde 3-phosphate | not | Streptococcus mutans | |
2.7.1.40 | additional information | no activation by glucose 1,6-diphosphate, 5'-IMP, 2',3'-AMP, 2',3'-GMP, 2',3'-UMP | Streptococcus mutans |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.40 | dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7 | Streptococcus mutans |
2.7.1.40 | phosphate restores activity of dialyzed, partially purified, not fully purified, enzyme preparations | Streptococcus mutans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | phosphate | at high concentrations; strong | Streptococcus mutans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.40 | additional information | - |
additional information | kinetic parameters | Streptococcus mutans | |
2.7.1.40 | 0.026 | - |
GDP | pH 7.5 | Streptococcus mutans | |
2.7.1.40 | 0.04 | 0.063 | phosphoenolpyruvate | pH 7.5 | Streptococcus mutans | |
2.7.1.40 | 0.049 | 0.1 | IDP | - |
Streptococcus mutans | |
2.7.1.40 | 0.22 | - |
phosphoenolpyruvate | in the presence of glucose 6-phosphate, at pH 7.5 | Streptococcus mutans | |
2.7.1.40 | 0.25 | 0.26 | GDP | UDP, at pH 7.5 | Streptococcus mutans | |
2.7.1.40 | 0.39 | - |
ADP | in the presence of glucose 6-phosphate, pH 7.5 | Streptococcus mutans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | Ca2+ | not | Streptococcus mutans | |
2.7.1.40 | divalent cation | requirement | Streptococcus mutans | |
2.7.1.40 | divalent cation | requires both a divalent and a monovalent cation | Streptococcus mutans | |
2.7.1.40 | K+ | requirement | Streptococcus mutans | |
2.7.1.40 | K+ | only together with activating divalent cation | Streptococcus mutans | |
2.7.1.40 | Mg2+ | requirement | Streptococcus mutans | |
2.7.1.40 | Mn2+ | requirement | Streptococcus mutans | |
2.7.1.40 | Mn2+ | only together with K+ | Streptococcus mutans | |
2.7.1.40 | monovalent cation | requirement | Streptococcus mutans | |
2.7.1.40 | monovalent cation | requires both a divalent and a monovalent cation | Streptococcus mutans | |
2.7.1.40 | additional information | absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this | Streptococcus mutans | |
2.7.1.40 | additional information | no activation by Li+ | Streptococcus mutans | |
2.7.1.40 | Na+ | not | Streptococcus mutans | |
2.7.1.40 | NH4+ | requirement | Streptococcus mutans | |
2.7.1.40 | NH4+ | can replace K+ | Streptococcus mutans | |
2.7.1.40 | NH4+ | only together with activating divalent cation | Streptococcus mutans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | 44000 | - |
4 * 44000, SDS-PAGE | Streptococcus mutans |
2.7.1.40 | 180000 | 190000 | gel filtration | Streptococcus mutans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.40 | Streptococcus mutans | - |
- |
- |
2.7.1.40 | Streptococcus mutans JC2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.40 | - |
Streptococcus mutans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.40 | cell culture | - |
Streptococcus mutans | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | additional information | - |
- |
Streptococcus mutans |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.40 | 4°C, in 40 mM potassium phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 3 months | Streptococcus mutans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.40 | ADP + phosphoenolpyruvate | - |
Streptococcus mutans | ATP + pyruvate | - |
? | |
2.7.1.40 | ADP + phosphoenolpyruvate | - |
Streptococcus mutans JC2 | ATP + pyruvate | - |
? | |
2.7.1.40 | CDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans | CTP + pyruvate | - |
? | |
2.7.1.40 | CDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | CTP + pyruvate | - |
? | |
2.7.1.40 | CDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans JC2 | CTP + pyruvate | - |
? | |
2.7.1.40 | CDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | CTP + pyruvate | - |
? | |
2.7.1.40 | GDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | GTP + pyruvate | - |
? | |
2.7.1.40 | GDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | GTP + pyruvate | - |
? | |
2.7.1.40 | IDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | ITP + pyruvate | - |
? | |
2.7.1.40 | TDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans | TTP + pyruvate | - |
? | |
2.7.1.40 | UDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | UTP + pyruvate | - |
? | |
2.7.1.40 | UDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | UTP + pyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.40 | tetramer | 4 * 44000, SDS-PAGE | Streptococcus mutans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | 6.8 | 7.7 | - |
Streptococcus mutans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | AMP | not | Streptococcus mutans | |
2.7.1.40 | GMP | not | Streptococcus mutans |