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Literature summary extracted from

  • Aust, A.; Yun, S.L.; Suelter, C.H.
    Pyruvate kinase from yeast (Saccharomyces cerevisiae) (1975), Methods Enzymol., 42C, 176-182.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Saccharomyces cerevisiae
2.7.1.40 0.049 0.1 IDP cosubstrate phosphoenolpyruvate Saccharomyces cerevisiae
2.7.1.40 0.099
-
 phosphoenolpyruvate pH 6.2, 30°C Saccharomyces cerevisiae
2.7.1.40 0.16
-
 ADP pH 6.2, 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Saccharomyces cerevisiae
2.7.1.40 K+ Km-value: 50 mM in the presence of fructose diphosphate Saccharomyces cerevisiae
2.7.1.40 Mg2+ requirement Saccharomyces pastorianus
2.7.1.40 Mg2+ requirement Saccharomyces cerevisiae
2.7.1.40 Mg2+ Km-value 2 mM in the presence of fructose diphosphate Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 190000
-
-
 Saccharomyces pastorianus
2.7.1.40 209400
-
 sedimentation equilibrium method Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate Saccharomyces cerevisiae allosteric enzyme ATP + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Saccharomyces cerevisiae
-
 Budweiser baker's yeast
-
2.7.1.40 Saccharomyces pastorianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 340
-
 pH 6.2, 30°C Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 2-4°C, in 90% saturated ammonium sulfate, at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Saccharomyces pastorianus ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Saccharomyces cerevisiae ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate allosteric enzyme Saccharomyces cerevisiae ATP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate
-
 Saccharomyces cerevisiae ITP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 50000-52000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 30
-
 assay at Saccharomyces pastorianus
2.7.1.40 30
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 additional information
-
 cold-labile enzyme Saccharomyces cerevisiae
2.7.1.40 additional information
-
 fructose 1,6-diphosphate enhances rate of inactivation, 25% glycerol and/or KCl and MgCl2 protect Saccharomyces cerevisiae