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Literature summary extracted from

  • Sabater, B.; Sebastian, J.; Asensio, C.
    Identification and properties of an inducible and highly specific fructokinase from Streptomyces violaceoruber (1972), Biochim. Biophys. Acta, 284, 414-420.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 D-fructose
-
 Streptomyces violaceoruber
2.7.1.4 MgADP- competitive Streptomyces violaceoruber
2.7.1.4 MgATP2-
-
 Streptomyces violaceoruber

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.2
-
 ATP ATP in form of MgATP2- Streptomyces violaceoruber
2.7.1.4 0.5
-
 D-fructose
-
 Streptomyces violaceoruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Co2+ can partially replace Mg2+ in activation Streptomyces violaceoruber
2.7.1.4 Mg2+ activation by free Mg2+ Streptomyces violaceoruber
2.7.1.4 additional information no activation by Mn2+ Streptomyces violaceoruber
2.7.1.4 additional information no activation by Cd2+ Streptomyces violaceoruber
2.7.1.4 additional information no activation by Cu2+ Streptomyces violaceoruber

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Streptomyces violaceoruber inducible enzyme ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Streptomyces violaceoruber
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4 400fold Streptomyces violaceoruber

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.4 mycelium
-
 Streptomyces violaceoruber
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 additional information
-
-
 Streptomyces violaceoruber
2.7.1.4 40
-
-
 Streptomyces violaceoruber

Storage Stability

EC Number Storage Stability Organism
2.7.1.4 -20°C, in presence of Mg2+ and dithiothreitol, 25% loss of activity after 6 months Streptomyces violaceoruber
2.7.1.4 2°C, pH 7, in presence of Mg2+ and dithioerythritol, 25% loss of activity after 2 weeks Streptomyces violaceoruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose specific for fructose Streptomyces violaceoruber ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose inducible enzyme Streptomyces violaceoruber ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 CTP + D-fructose 30% of the activity with ATP Streptomyces violaceoruber CDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 dATP + D-fructose 80% of the activity with ATP Streptomyces violaceoruber dADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose 50% of the activity with ATP Streptomyces violaceoruber GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ITP + D-fructose 60% of the activity with ATP Streptomyces violaceoruber IDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose 20% of the activity with ATP Streptomyces violaceoruber UDP + D-fructose 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 8.8 10 lengthy plateau in the alkaline zone Streptomyces violaceoruber

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.4 6
-
 1 h, 50% loss of activity Streptomyces violaceoruber

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.4 0.2
-
 MgATP2-
-
 Streptomyces violaceoruber