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Literature summary extracted from

  • Huo, X.; Viola, R.E.
    Functional group characterization of homoserine kinase from Escherichia coli (1996), Arch. Biochem. Biophys., 330, 373-379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.39 the combination of pTZ19u(ThrB) and the BL21(DE3) cell line is the best expression system with nearly 160 mg of enzyme produced in 4 liters of growth medium Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.39 diethyl dicarbonate
-
 Escherichia coli
2.7.1.39 L-homoserine substrate inhibition Escherichia coli
2.7.1.39 pyridoxal 5'-phosphate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.39 ATP + L-homoserine Escherichia coli key enzyme in the aspartate pathway of amino acid biosynthesis in E. coli ADP + O-phospho-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Escherichia coli ADP + O-phospho-L-homoserine
-
 ?
2.7.1.39 ATP + L-homoserine key enzyme in the aspartate pathway of amino acid biosynthesis in E. coli Escherichia coli ADP + O-phospho-L-homoserine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.39 6 8.5
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.39 additional information
-
 additional information
-
 Escherichia coli
2.7.1.39 0.1
-
 L-homoserine in 20% DMSO Escherichia coli
2.7.1.39 3
-
 L-homoserine aqueous sulution, pH 8 Escherichia coli