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Literature summary extracted from

  • Tanaka, R.D.; Schafer, B.L.; Lee, L.Y.; Freudenberger, J.S.; Mosley, S.T.
    Purification and regulation of mevalonate kinase from rat liver (1990), J. Biol. Chem., 265, 2391-2398.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.36 freezing of pure enzyme causes irreversible loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 farnesyl diphosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.27
-
 (RS)-mevalonate pH 7.0, 25°C Rattus norvegicus
2.7.1.36 1.75
-
 ATP pH 7.0, 25°C Rattus norvegicus
2.7.1.36 1.75
-
 ATP ATP in form of MgATP2- Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 39900
-
 2 * 39900, SDS-PAGE Rattus norvegicus
2.7.1.36 86000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + mevalonate Rattus norvegicus enzyme may play a key role in regulating cholesterol biosynthesis ADP + phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 ammonium sulfate, Blue-Sepharose, ammonium sulfate, Sephadex G-150, ATP-agarose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 31.67
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + mevalonate
-
 Rattus norvegicus ADP + phosphomevalonate
-
 ?
2.7.1.36 ATP + mevalonate enzyme may play a key role in regulating cholesterol biosynthesis Rattus norvegicus ADP + phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 dimer 2 * 39900, SDS-PAGE Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.36 0.0025
-
 farnesyl diphosphate pH 7.0, 25°C Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.36 Rattus norvegicus isoelectric focusing
-
 6.2