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Literature summary extracted from

  • Solovieva, I.M.; Tarasov, K.V.; Perumov, D.A.
    Main physicochemical features of monofunctional flavokinase from Bacillus subtilis (2003), Biochemistry, 68, 177-181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.26 7,8-dimethyl-10-(2'-hydroxyethyl)-isoalloxazine 0.5 mM, 10% inhibition Bacillus subtilis
2.7.1.26 7,8-dimethyl-10-(O-methylacetoxime)-isoaloxazine 0.5 mM, 34% inhibition Bacillus subtilis
2.7.1.26 Ca2+
-
 Bacillus subtilis
2.7.1.26 Co2+
-
 Bacillus subtilis
2.7.1.26 Lumiflavin 0.5 mM, 4% inhibition Bacillus subtilis
2.7.1.26 roseoflavin 0.4 mM, 8% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.112
-
 ATP pH 8, 37°C Bacillus subtilis
2.7.1.26 0.18
-
 riboflavin pH 8, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.26 Mg2+ required for maximal activity Bacillus subtilis
2.7.1.26 Zn2+ less effective in activation than Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.26 27250
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.26 ATP + riboflavin Bacillus subtilis key enzyme in flavin biosynthesis ADP + FMN
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Bacillus subtilis
-
 monofunctional enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Bacillus subtilis ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin key enzyme in flavin biosynthesis Bacillus subtilis ADP + FMN
-
 ?
2.7.1.26 CTP + riboflavin 50% of the activity with ATP Bacillus subtilis CDP + riboflavin 5'-phosphate
-
 ?
2.7.1.26 dATP + riboflavin
-
 Bacillus subtilis dADP + FMN
-
 ?
2.7.1.26 UTP + riboflavin 31% of the activity with ATP Bacillus subtilis UDP + FMN
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.26 52
-
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.26 8.5
-
-
 Bacillus subtilis