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Literature summary extracted from

  • Kearny, E.B.; Goldenberg, J.; Lipsick, J.; Perl, M.
    Flavokinase and FAD synthetase from Bacillus subtilis specific for reduced flavins (1979), J. Biol. Chem., 254, 9551-9557.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.26 10-(2'-Hydroxyethyl)-isoalloxazine 0.01 mM, 34% inhibition Bacillus subtilis
2.7.1.26 10-(4'-Carboxybutyl)-isoalloxazine 0.001 mM, 16% inhibition Bacillus subtilis
2.7.1.26 10-(5'-Hydroxypentyl)-isoalloxazine 0.01 mM, 38% inhibition Bacillus subtilis
2.7.1.26 2'-Thioriboflavin 0.01 mM, 59% inhibition Bacillus subtilis
2.7.1.26 3-methylriboflavin 0.01 mM, 5% inhibition Bacillus subtilis
2.7.1.26 7alpha-Methylriboflavin 0.01 mM, 95% inhibition Bacillus subtilis
2.7.1.26 Lumiflavin 0.01 mM, 38% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.0065
-
 ATP
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.26 Co2+ 0.1 mM, activation is about 65% of that with 0.2 mM Mg2+ Bacillus subtilis
2.7.1.26 Mg2+ 0.2 mM, Mg2+ or Zn2+ required Bacillus subtilis
2.7.1.26 Mn2+ activation is about 45% of that with 0.2 mM Mg2+ Bacillus subtilis
2.7.1.26 Zn2+ activation is about 90% of that with 0.2 mM Mg2+ Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.26
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.26 0.0129
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + 5-deazariboflavin
-
 Bacillus subtilis ADP + 5-deazariboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + riboflavin
-
 Bacillus subtilis ADP + FMN
-
 ?