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Literature summary extracted from

  • Merrill, A.H.; McCormick, D.B.
    Affinity chromatographic purification and properties of flavokinase (ATP:riboflavin 5-phosphotransferase) from rat liver (1980), J. Biol. Chem., 255, 1335-1338.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.26 enzyme is inactivated by freezing and thawing unless both riboflavin and 20% glycerol are added Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.26 1-Deazariboflavin
-
 Rattus norvegicus
2.7.1.26 10-(5'-Hydroxypentyl)flavin
-
 Rattus norvegicus
2.7.1.26 10-(Hydroxyethyl)flavin
-
 Rattus norvegicus
2.7.1.26 3-Deazariboflavin
-
 Rattus norvegicus
2.7.1.26 5-Deazariboflavin
-
 Rattus norvegicus
2.7.1.26 8-Aminoriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Diethylaminoriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Ethoxyriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Ethylaminoriboflavin
-
 Rattus norvegicus
2.7.1.26 8-hydroxyriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Methoxyriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Methylaminoriboflavin
-
 Rattus norvegicus
2.7.1.26 8-Methylethylaminoriboflavin
-
 Rattus norvegicus
2.7.1.26 Ba2+
-
 Rattus norvegicus
2.7.1.26 Fe2+
-
 Rattus norvegicus
2.7.1.26 lumichrome
-
 Rattus norvegicus
2.7.1.26 Lumiflavin
-
 Rattus norvegicus
2.7.1.26 riboflavin 5'-phosphate
-
 Rattus norvegicus
2.7.1.26 Sr2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.01
-
 riboflavin pH 8.0, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.26 Mg2+ activates Rattus norvegicus
2.7.1.26 Zn2+ activation is 1.8times of that with Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.26 27000
-
 gel filtration Rattus norvegicus
2.7.1.26 28000
-
 1 * 28000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.26
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.26 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.26 0.165
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.26 4°C, 50% loss of activity after 2 d when the enzyme is stored in buffer alone, complete protection by 0.01 mM riboflavin Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + 10-(D-allo)flavin 30% of the activity with riboflavin Rattus norvegicus ADP + 10-(D-allo)flavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 10-(L-arabo)flavin 25% of the activity with riboflavin Rattus norvegicus ADP + 10-(L-arabo)flavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 2-thioriboflavin 60% of the activity with riboflavin Rattus norvegicus ADP + 2-thioriboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 8-demethylriboflavin 35% of the activity with riboflavin Rattus norvegicus ADP + 8-demethylriboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 8-dimethylamino-8-demethylriboflavin 70% of the activity with riboflavin Rattus norvegicus ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + D-erythroflavin 10-(D-erythro)flavin, 33% of the activity with riboflavin Rattus norvegicus ADP + D-erythroflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + riboflavin
-
 Rattus norvegicus ADP + FMN
-
 ?
2.7.1.26 dATP + riboflavin
-
 Rattus norvegicus dADP + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.26 monomer 1 * 28000, SDS-PAGE Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.26 0.007
-
 Lumiflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.007
-
 10-(Hydroxyethyl)flavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.008
-
 10-(5'-Hydroxypentyl)flavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.01
-
 lumichrome pH 8, 37°C Rattus norvegicus
2.7.1.26 0.015
-
 8-Methoxyriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.016
-
 8-Ethoxyriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.018
-
 riboflavin 5'-phosphate pH 8, 37°C Rattus norvegicus
2.7.1.26 0.02
-
 8-Diethylaminoriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.021
-
 1-Deazariboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.03
-
 8-Methylethylaminoriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.175
-
 8-Ethylaminoriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.25
-
 8-Aminoriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.275
-
 5-Deazariboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.41
-
 3-Deazariboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.47
-
 8-Methylaminoriboflavin pH 8, 37°C Rattus norvegicus
2.7.1.26 0.5
-
 8-hydroxyriboflavin pH 8, 37°C Rattus norvegicus