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Literature summary extracted from

  • Lee, S.; Leustek, T.
    APS kinase from Arabidopsis thaliana: genomic organization, expression, and kinetic analysis of the recombinant enzyme (1998), Biochem. Biophys. Res. Commun., 247, 171-175.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expression in Escherichia coli as fusion protein with glutathione-S-transferase or maltose binding protein Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate substrate inhibition above 0.01 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.0036
-
 adenosine 5'-phosphosulfate 25°C Arabidopsis thaliana
2.7.1.25 1.9
-
 ATP 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.25 chloroplast localization is suggested by an in vitro chloroplast import assay Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 29786
-
 x * 29786, deduced from nucleotide sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Arabidopsis thaliana Q43295
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 leaf APS kinase mRNA Arabidopsis thaliana
-
2.7.1.25 root APS kinase mRNA Arabidopsis thaliana
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.25 -70°C, recombinant APS kinase fusion protein, 1 year, no loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 ? x * 29786, deduced from nucleotide sequence Arabidopsis thaliana