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Literature summary extracted from

  • Lerner, F.; Niere, M.; Ludwig, A.; Ziegler, M.
    Structural and functional characterization of human NAD kinase (2001), Biochem. Biophys. Res. Commun., 288, 69-74.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 cDNA is amplified and functionally overexpressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 3-acetyl-pyridine adenine dinucleotide 1 mM, 30% inhibition Homo sapiens
2.7.1.23 3-aldehyde-pyridine adenine dinucleotide 1 mM, 32% inhibition Homo sapiens
2.7.1.23 nicotinamide guanine dinucleotide 1 mM, 23% inhibition Homo sapiens
2.7.1.23 nicotinamide hypoxanthine dinucleotide 1 mM, 24% inhibition Homo sapiens
2.7.1.23 nicotinic acid adenine dinucleotide phosphate 1 mM, 37% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.54
-
 NAD+ 30°C Homo sapiens
2.7.1.23 3.3
-
 ATP 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Mg2+ optimal concentration is 10 mM. At 1 mM, 29% of the activation with 1 mM Zn2+ Homo sapiens
2.7.1.23 Mn2+ 1 mM, 83% of the activation with 1 mM Zn2+ Homo sapiens
2.7.1.23 Zn2+ 1 mM, highest activation of divalent metal ions tested Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 49228
-
 4 * 49228, calculation from nucleotide sequence Homo sapiens
2.7.1.23 200000
-
 gel filtration Bos taurus
2.7.1.23 200000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.23 ATP + NAD+ Homo sapiens synthesis of NADP+ ADP + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Bos taurus
-
-
-
2.7.1.23 Homo sapiens O95544
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23 partial Bos taurus
2.7.1.23 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.23 fibroblast
-
 Homo sapiens
-
2.7.1.23 liver
-
 Bos taurus
-
2.7.1.23 placenta
-
 Homo sapiens
-
2.7.1.23 skeletal muscle no expression of NAD kinase in skeletal muscle Homo sapiens
-
2.7.1.23 small intestine little expression Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 6.7
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Bos taurus ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Homo sapiens ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ synthesis of NADP+ Homo sapiens ADP + NADP+
-
 ?
2.7.1.23 GTP + NAD+ 7% of the activity with ATP Homo sapiens GDP + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 hexamer 4 * 49228, calculation from nucleotide sequence Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.23 55
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 60
-
 5 min, less than 5% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 7.8
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.23 5.5
-
 rapid inactivation below Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.23 NAD+
-
 Bos taurus
2.7.1.23 NAD+
-
 Homo sapiens