Literature summary extracted from

Wang, L.; Eriksson, S.
Cloning and characterization of full-length mouse thymidine kinase 2: the N-terminal sequence directs import of the precursor protein into mitochondria (2000), Biochem. J., 351, 469-476.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.21	0.0034	-	thymidine	mTK2 pH 7.6	Mus musculus
2.7.1.21	0.0049	-	thymidine	mTK2-39 pH 7.6	Mus musculus
2.7.1.21	0.0073	-	thymidine	mTK2-55 pH 7.6	Mus musculus
2.7.1.21	0.0115	-	deoxycytidine	mTK2-55 pH 7.6	Mus musculus
2.7.1.21	0.0236	-	deoxycytidine	mTK2-39 pH 7.6	Mus musculus
2.7.1.21	0.0251	-	deoxycytidine	mTK2 pH 7.6	Mus musculus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.1.21	30000	-	gel filtration, two enzyme forms of MW 30000 and 60000	Mus musculus
2.7.1.21	60000	-	gel filtration, two enzyme forms of MW 30000 and 60000	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.21	Mus musculus	Q9R088	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.21	3 forms	Mus musculus

Storage Stability

EC Number	Storage Stability	Organism
2.7.1.21	4°C, MTK1-39 and mTK2-55 are stable for up to 3 weeks, mTK2 is less stable	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.21	ATP + 2'-deoxycytidine	-	Mus musculus	ADP + 2'-deoxycytidine 5'-phosphate	-	?
2.7.1.21	ATP + 2'-deoxyuridine	-	Mus musculus	ADP + 2'-deoxyuridine 5'-phosphate	-	?
2.7.1.21	ATP + thymidine	-	Mus musculus	ADP + dTMP	-	?
2.7.1.21	additional information	overview: nucleoside analogs	Mus musculus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.1.21	dimer	-	Mus musculus

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Release 2023.1 (January 2023)