BRENDA - Enzyme Database show

Cloning and characterization of full-length mouse thymidine kinase 2: the N-terminal sequence directs import of the precursor protein into mitochondria

Wang, L.; Eriksson, S.; Biochem. J. 351, 469-476 (2000)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0034
-
thymidine
mTK2 pH 7.6
Mus musculus
2.7.1.21
0.0049
-
thymidine
mTK2-39 pH 7.6
Mus musculus
2.7.1.21
0.0073
-
thymidine
mTK2-55 pH 7.6
Mus musculus
2.7.1.21
0.0115
-
deoxycytidine
mTK2-55 pH 7.6
Mus musculus
2.7.1.21
0.0236
-
deoxycytidine
mTK2-39 pH 7.6
Mus musculus
2.7.1.21
0.0251
-
deoxycytidine
mTK2 pH 7.6
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.21
30000
-
gel filtration, two enzyme forms of MW 30000 and 60000
Mus musculus
2.7.1.21
60000
-
gel filtration, two enzyme forms of MW 30000 and 60000
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Mus musculus
Q9R088
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.21
3 forms
Mus musculus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.21
4°C, MTK1-39 and mTK2-55 are stable for up to 3 weeks, mTK2 is less stable
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641170
Mus musculus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 2'-deoxyuridine
-
641170
Mus musculus
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
-
641170
Mus musculus
ADP + dTMP
-
-
-
?
2.7.1.21
additional information
overview: nucleoside analogs
641170
Mus musculus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.21
dimer
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0034
-
thymidine
mTK2 pH 7.6
Mus musculus
2.7.1.21
0.0049
-
thymidine
mTK2-39 pH 7.6
Mus musculus
2.7.1.21
0.0073
-
thymidine
mTK2-55 pH 7.6
Mus musculus
2.7.1.21
0.0115
-
deoxycytidine
mTK2-55 pH 7.6
Mus musculus
2.7.1.21
0.0236
-
deoxycytidine
mTK2-39 pH 7.6
Mus musculus
2.7.1.21
0.0251
-
deoxycytidine
mTK2 pH 7.6
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.21
30000
-
gel filtration, two enzyme forms of MW 30000 and 60000
Mus musculus
2.7.1.21
60000
-
gel filtration, two enzyme forms of MW 30000 and 60000
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
3 forms
Mus musculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.21
4°C, MTK1-39 and mTK2-55 are stable for up to 3 weeks, mTK2 is less stable
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641170
Mus musculus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 2'-deoxyuridine
-
641170
Mus musculus
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
-
641170
Mus musculus
ADP + dTMP
-
-
-
?
2.7.1.21
additional information
overview: nucleoside analogs
641170
Mus musculus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.21
dimer
-
Mus musculus