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Literature summary extracted from

  • Henderson, J.F.; Mikoshiba, A.; Chu, S.Y.; Caldwell, I.C.
    Kinetic studies of adenosine kinase from Ehrlich ascites tumor cells (1972), J. Biol. Chem., 247, 1972-1975.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 6-Methylmercaptopurine riboside and analogs Mus musculus
2.7.1.20 6-methylmercaptopurine riboside phosphate product inhibition, competitive with respect to ATP and noncompetitive with respect to 6-methylmercaptopurine ribonucleoside Mus musculus
2.7.1.20 7-beta-D-ribofuranosyl-pyrrolo-(2,3-d)-pyrimidine competitive with respect to 6-methylmercaptopurine riboside and noncompetitive with respect to ATP Mus musculus
2.7.1.20 adenosine competitive with respect to 6-methylmercaptopurine riboside Mus musculus
2.7.1.20 ADP noncompetitive with respect to ATP and 6-methylmercaptopurine ribonucleoside at low concentrations of the substrates; product inhibition Mus musculus
2.7.1.20 ATP-analogs
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 additional information
-
 additional information kinetic study Mus musculus
2.7.1.20 0.01
-
 6-Methylmercaptopurine riboside pH 6.0, 37ºC Mus musculus
2.7.1.20 0.025
-
 ATP pH 6.0, 37ºC Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 ammonium sulfate fractionation and gel filtration, 15-20fold purification Mus musculus
2.7.1.20 partial Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.20 ATP + adenosine = ADP + AMP mechanism Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -20°C, partially purified, 1 year Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 6-methylmercaptopurine riboside
-
 Mus musculus ADP + 6-methylmercaptopurine-D-riboside 5'-phosphate
-
 ?
2.7.1.20 ATP + adenosine
-
 Mus musculus ADP + AMP
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.20 0.015
-
 7-beta-D-ribofuranosyl-pyrrolo-(2,3-d)-pyrimidine pH 6.0, 37ºC Mus musculus
2.7.1.20 0.06
-
 ADP pH 6.0, 37ºC Mus musculus
2.7.1.20 0.16
-
 6-methylmercaptopurine riboside phosphate pH 6.0, 37ºC Mus musculus
2.7.1.20 0.26
-
 adenosine pH 6.0, 37ºC Mus musculus