BRENDA - Enzyme Database

Inhibition of adenosine and thymidylate kinases by bisubstrate analogs

Bone, R.; Cheng, Y.C.; Wolfenden, R.; J. Biol. Chem. 261, 16410-16413 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.20
adenosine-5'-pentaphospho-5'-adenosine
-
Homo sapiens
2.7.1.20
adenosine-5'-tetraphospho-5'-adenosine
strong inhibition
Homo sapiens
2.7.1.20
Bisadenylyl polyphosphates
-
Homo sapiens
2.7.4.9
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
-
Homo sapiens
2.7.4.9
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
-
Homo sapiens
2.7.4.9
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.20
Mg2+
optimal at Mg: ATP ratio 1; requirement
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.20
Homo sapiens
-
-
-
2.7.4.9
Homo sapiens
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.20
ATP + adenosine = ADP + AMP
mechanism
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
liver
-
Homo sapiens
-
2.7.4.9
blast cell
peripheral blast cell obtained from acute myelocytic leukemia patients
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
-
641085
Homo sapiens
ADP + AMP
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.20
37
-
assay at
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.20
0.00003
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.4, 37C
Homo sapiens
2.7.1.20
0.000073
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.4, 37C
Homo sapiens
2.7.4.9
0.00018
-
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0002
-
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
2.7.4.9
0.00047
-
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0006
-
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
2.7.4.9
0.0175
-
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0209
-
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.20
adenosine-5'-pentaphospho-5'-adenosine
-
Homo sapiens
2.7.1.20
adenosine-5'-tetraphospho-5'-adenosine
strong inhibition
Homo sapiens
2.7.1.20
Bisadenylyl polyphosphates
-
Homo sapiens
2.7.4.9
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
-
Homo sapiens
2.7.4.9
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
-
Homo sapiens
2.7.4.9
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.20
0.00003
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.4, 37C
Homo sapiens
2.7.1.20
0.000073
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.4, 37C
Homo sapiens
2.7.4.9
0.00018
-
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0002
-
P1-(Adenosine 5')-P6-(thymidine 5')-hexaphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
2.7.4.9
0.00047
-
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0006
-
P1-(adenosine 5')-P5-(thymidine 5')-pentaphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
2.7.4.9
0.0175
-
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
pH 7.5, 37C, with dTMP as variable substrate and ATP as cosubstrate
Homo sapiens
2.7.4.9
0.0209
-
P1-(adenosine 5')-P4-(thymidine 5')-tetraphosphate
pH 7.5, 37C, with ATP as variable substrate and dTMP as cosubstrate
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.20
Mg2+
optimal at Mg: ATP ratio 1; requirement
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
liver
-
Homo sapiens
-
2.7.4.9
blast cell
peripheral blast cell obtained from acute myelocytic leukemia patients
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
-
641085
Homo sapiens
ADP + AMP
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.20
37
-
assay at
Homo sapiens