BRENDA - Enzyme Database show

Purine nucleoside kinases in human T- and B-lymphoblasts

Yamada, Y.; Goto, H.; Ogasawara, N.; Biochim. Biophys. Acta 761, 34-40 (1983)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.20
bovine serum albumin stabilizes
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
adenosine arabinoside
weak, not with deoxyadenosine as substrate
Homo sapiens
2.7.1.74
CTP
strong, ATP as substrate
Homo sapiens
2.7.1.74
cytidine
weak
Homo sapiens
2.7.1.74
cytosine arabinoside
ATP as substrate; deoxycytidine as substrate; strong, deoxyadenosine as substrate
Homo sapiens
2.7.1.74
dATP
strong
Homo sapiens
2.7.1.74
dCTP
-
Homo sapiens
2.7.1.74
deoxyadenosine
-
Homo sapiens
2.7.1.74
deoxycytidine
deoxyadenosine as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxyguanosine as substrate, cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
dGTP
ATP as substrate
Homo sapiens
2.7.1.74
dTTP
ATP as substrate; weak
Homo sapiens
2.7.1.74
GTP
not, deoxyadenosine as substrate; weak, ATP and deoxycytidine as substrate
Homo sapiens
2.7.1.74
additional information
little or no inhibition by AMP, dTMP, adenosine, guanosine
Homo sapiens
2.7.1.74
Nucleosides
overview, ATP and deoxyadenosine or deoxycytidine as substrate
Homo sapiens
2.7.1.74
UTP
ATP as substrate; not with deoxyadenosine as substrate
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
-
Homo sapiens
2.7.1.74
0.15
-
deoxyadenosine
pH 8.0
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.20
cytosol
-
Homo sapiens
5829
-
2.7.1.74
viral nucleocapsid
Epstein-Barr-Virus
Homo sapiens
19013
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.20
Homo sapiens
-
-
-
2.7.1.74
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
lymphoblast
T-lymphoblast cell lines MILT 4F, CCRF CEM and RPMI 8402, B-lymphoblast cell lines BALL 1 and EBV (Epstein-Barr-virus)-transformed B-lymphoblast
Homo sapiens
-
2.7.1.74
BALL-1 cell
-
Homo sapiens
-
2.7.1.74
CCRF-CEM cell
-
Homo sapiens
-
2.7.1.74
lymphoblast
T-lymphoblasts, cell lines MOLT 4F, CCRF, CEM and RPMI 8402, B-lymphoblasts cell lines BALL 1 and EBV (Epstein-Barr-virus) transformed B-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4F cell
-
Homo sapiens
-
2.7.1.74
RPMI-8402 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
-
641079
Homo sapiens
ADP + AMP
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
641079
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641079
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641079
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641079
Homo sapiens
NDP + dGMP
-
-
-
?
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.20
bovine serum albumin stabilizes
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
adenosine arabinoside
weak, not with deoxyadenosine as substrate
Homo sapiens
2.7.1.74
CTP
strong, ATP as substrate
Homo sapiens
2.7.1.74
cytidine
weak
Homo sapiens
2.7.1.74
cytosine arabinoside
ATP as substrate; deoxycytidine as substrate; strong, deoxyadenosine as substrate
Homo sapiens
2.7.1.74
dATP
strong
Homo sapiens
2.7.1.74
dCTP
-
Homo sapiens
2.7.1.74
deoxyadenosine
-
Homo sapiens
2.7.1.74
deoxycytidine
deoxyadenosine as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxyguanosine as substrate, cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
dGTP
ATP as substrate
Homo sapiens
2.7.1.74
dTTP
ATP as substrate; weak
Homo sapiens
2.7.1.74
GTP
not, deoxyadenosine as substrate; weak, ATP and deoxycytidine as substrate
Homo sapiens
2.7.1.74
additional information
little or no inhibition by AMP, dTMP, adenosine, guanosine
Homo sapiens
2.7.1.74
Nucleosides
overview, ATP and deoxyadenosine or deoxycytidine as substrate
Homo sapiens
2.7.1.74
UTP
ATP as substrate; not with deoxyadenosine as substrate
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
-
Homo sapiens
2.7.1.74
0.15
-
deoxyadenosine
pH 8.0
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.20
cytosol
-
Homo sapiens
5829
-
2.7.1.74
viral nucleocapsid
Epstein-Barr-Virus
Homo sapiens
19013
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
lymphoblast
T-lymphoblast cell lines MILT 4F, CCRF CEM and RPMI 8402, B-lymphoblast cell lines BALL 1 and EBV (Epstein-Barr-virus)-transformed B-lymphoblast
Homo sapiens
-
2.7.1.74
BALL-1 cell
-
Homo sapiens
-
2.7.1.74
CCRF-CEM cell
-
Homo sapiens
-
2.7.1.74
lymphoblast
T-lymphoblasts, cell lines MOLT 4F, CCRF, CEM and RPMI 8402, B-lymphoblasts cell lines BALL 1 and EBV (Epstein-Barr-virus) transformed B-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4F cell
-
Homo sapiens
-
2.7.1.74
RPMI-8402 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
-
641079
Homo sapiens
ADP + AMP
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
641079
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641079
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641079
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641079
Homo sapiens
NDP + dGMP
-
-
-
?