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Literature summary extracted from

  • Lindberg, B.; Klenow, H.; Hansen, K.
    Some properties of partially purified mammalian adenosine kinase (1967), J. Biol. Chem., 242, 350-356.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 ATP 0.7 mM with 0.5 mM Mg2+, 0.9 mM with 1 mM Mg2+ and 1.5 mM with 2 mM Mg2+ Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.1.20 GSH, 2-mercaptoethanol, Mg2+ and adenosine do not stabilize Oryctolagus cuniculus
2.7.1.20 rapid inactivation in aqueous solution Oryctolagus cuniculus
2.7.1.20 unstable upon ammonium sulfate fractionation or adsorption on aluminium oxide or calcium phosphate gel Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 6-mercaptopurine riboside no inhibition Oryctolagus cuniculus
2.7.1.20 additional information
-
 Mus musculus
2.7.1.20 additional information inosine, aminonucleoside of puromycin, 2'-deoxyadenosine N1-oxide, 3'-deoxyadenosine N1-oxide, 8-aza-2'-deoxyadenosine, 2-aminopurine-2'-deoxyriboside, thioguanosine, psicofuranine, N6-methyl-2'-deoxyadenosine, N1-methyladenosine do not inhibit the enzyme activity Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.14
-
 arabinofuranosyladenine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.14
-
 4'-thioadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.15
-
 xylofuranosyladenine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.16
-
 adenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.16
-
 N6-methyladenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.18
-
 2'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.34
-
 2'-amino-2',3'-dideoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.35
-
 3'-deoxyadenosine pH 5.8, 25ºC, natural Oryctolagus cuniculus
2.7.1.20 0.4
-
 tubercidin pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.41
-
 adenosine-N1-oxide pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.42
-
 2,6-diaminopurine riboside pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.43
-
 2-fluoroadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.46
-
 3'-deoxyadenosine pH 5.8, 25ºC, natural Mus musculus
2.7.1.20 0.47
-
 3'-deoxyadenosine pH 5.8, 25ºC, synthetic Oryctolagus cuniculus
2.7.1.20 0.54
-
 N6-methyl-3'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.55
-
 6-Chloropurine riboside pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.58
-
 adenosine pH 5.8, 25ºC Mus musculus
2.7.1.20 0.61
-
 3'-amino-3'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.78
-
 purine riboside pH 5.8, 25ºC Oryctolagus cuniculus
2.7.1.20 0.8
-
 Toyocamycin pH 5.8, 25ºC Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.20 cytosol
-
 Mus musculus 5829
-
2.7.1.20 cytosol
-
 Oryctolagus cuniculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ requirement Mus musculus
2.7.1.20 Mg2+ requirement Oryctolagus cuniculus
2.7.1.20 Mg2+ optimal at Mg: ATP ratio 1 Mus musculus
2.7.1.20 Mg2+ optimal at Mg: ATP ratio 1 Oryctolagus cuniculus
2.7.1.20 Mg2+ 0.5 mM Mus musculus
2.7.1.20 Mg2+ 0.5 mM Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 23000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Mus musculus
-
-
-
2.7.1.20 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 chromatography on Sephadex G-100 Mus musculus
2.7.1.20 chromatography on Sephadex G-100 Oryctolagus cuniculus
2.7.1.20 partial Mus musculus
2.7.1.20 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 Ehrlich ascites carcinoma cell
-
 Mus musculus
-
2.7.1.20 liver
-
 Mus musculus
-
2.7.1.20 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 0.06 0.1
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -18°C, in 20% glycerol, at least 6 months Mus musculus
2.7.1.20 -18°C, in 20% glycerol, at least 6 months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-amino-2',3'-dideoxyadenosine
-
 Oryctolagus cuniculus ADP + 2'-amino-2',3'-dideoxyadenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Oryctolagus cuniculus ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + 2,6-diaminopurine riboside
-
 Oryctolagus cuniculus ADP + 2,6-diaminopurine riboside 5'-phosphate
-
 ?
2.7.1.20 ATP + 2-fluoroadenosine
-
 Oryctolagus cuniculus ADP + 2-fluoroadenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + 3'-amino-3'-deoxyadenosine
-
 Oryctolagus cuniculus ADP + 3'-amino-3'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + 4'-thioadenosine
-
 Oryctolagus cuniculus ADP + 4'-thioadenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + 6-chloropurine riboside
-
 Oryctolagus cuniculus ADP + 6-chloropurine riboside 5'-phosphate
-
 ?
2.7.1.20 ATP + 9-[beta-D-ribofuranosyl]-purine
-
 Oryctolagus cuniculus ADP + 9-[beta-D-ribofuranosyl]-purine 5'-phosphate
-
 ?
2.7.1.20 ATP + adenosine MgATP2- is the true substrate Mus musculus ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine MgATP2- is the true substrate Oryctolagus cuniculus ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine-1-N-oxide
-
 Oryctolagus cuniculus ADP + adenosine-1-N-oxide 5'-phosphate
-
 ?
2.7.1.20 ATP + arabinofuranosyladenine
-
 Oryctolagus cuniculus ADP + arabinofuranosyladenine 5'-phosphate
-
 ?
2.7.1.20 ATP + cordycepin
-
 Mus musculus ADP + cordycepin 5'-phosphate
-
 ?
2.7.1.20 ATP + cordycepin
-
 Oryctolagus cuniculus ADP + cordycepin 5'-phosphate
-
 ?
2.7.1.20 ATP + N6-methyl-3'-deoxyadenosine
-
 Oryctolagus cuniculus ADP + N6-methyl-3'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + N6-methyladenosine
-
 Oryctolagus cuniculus ADP + N6-methyladenosine 5'-phosphate
-
 ?
2.7.1.20 ATP + toyocamycin
-
 Oryctolagus cuniculus ADP + toyocamycin 5'-phosphate
-
 ?
2.7.1.20 ATP + tubercidin
-
 Oryctolagus cuniculus ADP + tubercidin 5'-phosphate
-
 ?
2.7.1.20 ATP + xylofuranosyladenine
-
 Oryctolagus cuniculus ADP + xylofuranosyladenine 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.20 37
-
-
 Mus musculus
2.7.1.20 37
-
 t1/2: 45 min, at pH 7.4 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 5.8
-
 in Tris-maleate or phosphate buffer Oryctolagus cuniculus
2.7.1.20 6.5
-
 citrate-phosphate buffer Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.20 7.4
-
 t1/2: 45 min at 37°C Mus musculus
2.7.1.20 7.4
-
 t1/2: 45 min at 37°C Oryctolagus cuniculus