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Literature summary extracted from

  • Siebert, K.; Schobert, P.; Bowien, B.
    Purification, some catalytic and molecular properties of phosphoribulokinase from Alcaligenes eutrophus (1981), Biochim. Biophys. Acta, 658, 35-44.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.19 Ca2+ can partially replace Mg2+ Cupriavidus necator
2.7.1.19 Co2+ can partially replace Mg2+ Cupriavidus necator
2.7.1.19 Mg2+ required Cupriavidus necator
2.7.1.19 Mn2+ can partially replace Mg2+ Cupriavidus necator
2.7.1.19 additional information not activated by Ni2+ Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 33000
-
 8 * 33000, subunits of identical size, SDS-PAGE Cupriavidus necator
2.7.1.19 252000 256000 sedimentation equilibrium centrifugation, gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Cupriavidus necator
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 homogeneity Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 additional information
-
-
 Cupriavidus necator
2.7.1.19 7.59
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 0-4°C, no significant loss of activity after 1 month Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-fructose 6-phosphate + ATP 3.6% of ribulose 5-phosphate activity Cupriavidus necator ?
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Cupriavidus necator D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Cupriavidus necator D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Cupriavidus necator D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 sedoheptulose 7-phosphate + ATP 2.5% of ribulose 5-phosphate activity Cupriavidus necator ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 octamer 8 * 33000, subunits of identical size, SDS-PAGE Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.19 8.6
-
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.19 NADH activation Cupriavidus necator