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Literature summary extracted from

  • Rippel, S.; Bowien, B.
    Phosphoribulokinase from Rhodospseudomonas acidophila (1984), Arch. Microbiol., 139, 207-212.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 NADH strong activator Rhodoblastus acidophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 phosphoenolpyruvate 50% inhibition at 0.32 mM Rhodoblastus acidophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.19 Mg2+ required Rhodoblastus acidophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 248000
-
 sucrose density gradient centrifugation Rhodoblastus acidophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Rhodoblastus acidophilus
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP Rhodoblastus acidophilus 1150
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Rhodoblastus acidophilus
-
 strain 01150
-
2.7.1.19 Rhodoblastus acidophilus 1150
-
 strain 01150
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 homogeneity Rhodoblastus acidophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 111.2
-
-
 Rhodoblastus acidophilus

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 0-4°C, 100 mM imidazole-HCl buffer, pH 7.0, 10 mM MgCl2, 1 mM EDTA, 1 mM dithioerythritol, 3 months Rhodoblastus acidophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by TTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by TTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.19 7.6
-
 presence of NADH Rhodoblastus acidophilus
2.7.1.19 8.4
-
 absence of NADH Rhodoblastus acidophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.19 NADH activation Rhodoblastus acidophilus
2.7.1.19 NADH pH-dependent Rhodoblastus acidophilus