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Literature summary extracted from

  • Kolesnick, R.N.; Hemer, M.R.
    Characterization of a ceramide kinase activity from human leukemia (HL-60) cells. Separation from diacylglycerol kinase activity (1990), J. Biol. Chem., 265, 18803-18808.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.138 20% glycerol and 0.05 mM ATP stabilize during solubilization Homo sapiens
2.7.1.138 solubilized enzyme loses activity overnight at 4°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.138 additional information not inhibited by 1,2-diacylglycerol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.138 0.025
-
 ATP pH 7.0, 22°C Homo sapiens
2.7.1.138 0.045
-
 ceramide pH 7.0, 22°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.138 microsome membrane Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Ca2+ required for activity, 60 nM - 3 mM, 20fold linear increase in activity, progressive loss of activity at higher concentrations Homo sapiens
2.7.1.138 Mg2+ 0.3-30 mM stimulates Homo sapiens
2.7.1.138 additional information no stimulation by Mn2+, Ba2+, Cd2+ and Zn2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 leukemia cell line cell line HL-60 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.138 -70°C, 48 h, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide no activity with 1,2-diacylglycerol Homo sapiens ADP + ceramide 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 6.5 7
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.138 5.5 8 approx. 20% of maximal activity at pH 5.5 and pH 8.0 Homo sapiens