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Literature summary extracted from

  • Arcaro, A.; Volinia, S.; Zvelebil, M.J.; Stein, R.; Watton, S.J.; Layton, M.J.; Gout, I.; Ahmadi, K.; Downward, J.; Waterfield, M.D.
    Human phosphoinositide 3-kinase C2b, the role of calcium and the C2 domain in enzyme activity (1998), J. Biol. Chem., 273, 33082-33090.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.137 class II phosphoinositide 3-kinase with a C2 domain, cloned from a U937 monocyte cDNA library, expression in mammalian and insect cells Homo sapiens
2.7.1.137 class II phosphoinositide 3-kinase, PI 3-kinase C2b, with a C2 domain is cloned from a U937 monocyte cDNA library and expressed in mammalian and insect cells Homo sapiens
2.7.1.154 Glu-tagged PI 3-kinase C2beta protein is expressed in both HEK293 cells and Sf9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.137 Wortmannin at nanomolar levels Homo sapiens
2.7.1.154 Wortmannin IC50: 1.6 nM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.154 0.12
-
 ATP pH 7.4 Homo sapiens
2.7.1.154 0.12
-
 ATP ATP in form of CaATP2- Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.154 additional information the recombinant enzyme is localized mainly in the low density microsomal fraction, with a small amount being present in the plasma membrane and the cytosolic fraction Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.137 Ca2+ can use either MgATP2- or CaATP2- Homo sapiens
2.7.1.137 Mg2+ can use either MgATP2- or CaATP2- Homo sapiens
2.7.1.154 Ca2+ phosphorylation of phosphatidylinositol but not phosphatidylinositol 4-phosphate in presence of Ca2+ Homo sapiens
2.7.1.154 Mg2+ phosphorylation of phosphatidylinositol and phosphatidylinositol 4-phosphate in presence of Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.154 182000
-
 x * 182000, calculation from nucleotide sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Homo sapiens
-
 class II phosphoinositide 3-kinase, PI 3-kinase C2b
-
2.7.1.137 Homo sapiens O00750
-
-
2.7.1.154 Homo sapiens O00750 PI3K-C2beta
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.137 cell culture U937 monocyte Homo sapiens
-
2.7.1.137 cell culture U937 monocytes Homo sapiens
-
2.7.1.137 monocyte
-
 Homo sapiens
-
2.7.1.137 monocyte U937 monocyte Homo sapiens
-
2.7.1.154 U-937 cell monocyte Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidylinositol
-
 Homo sapiens ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.137 ATP + phosphatidylinositol 4,5-bisphosphate no activity Homo sapiens ADP + phosphatidylinositol 3,4,5-trisphosphate
-
 ?
2.7.1.137 ATP + phosphatidylinositol 4-phosphate
-
 Homo sapiens ADP + phosphatidylinositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate at 10% of the activity with phosphatidylinositol, phosphorylation only in presence of Mg2+, not in presence of Ca2+ Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + phosphatidylinositol
-
 Homo sapiens ADP + phosphatidylinositol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.154 ? x * 182000, calculation from nucleotide sequence Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.154 0.0000016
-
 IC50: 1.6 nM Homo sapiens Wortmannin