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Literature summary extracted from

  • O'Hara, K.; Kanda,T.; Ohmiya, K.; Ebisu, T.; Kono, M.
    Purification and characterization of macrolide 2'-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin (1989), Antimicrob. Agents Chemother., 33, 1354-1357.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.136 erythromycin crude cell extracts from cells treated with erythromycin show increased activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.136 CoSO4 1 mM, 51% inhibition Escherichia coli
2.7.1.136 CuSO4 1 mM, 52% inhibition Escherichia coli
2.7.1.136 EDTA 10 mM, 100% inhibition Escherichia coli
2.7.1.136 FeSO4 1 mM, 66% inhibition Escherichia coli
2.7.1.136 Iodine 1 mM, 100% inhibition Escherichia coli
2.7.1.136 additional information 1 mM p-chloromercuribenzoic acid or urea are no inhibitors, MphR(A) is a repressor protein that binds to the promoter of the mph(A) gene and negatively regulates the enzyme expression Escherichia coli
2.7.1.136 ZnSO4 1 mM, 93% inhibition Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.136 intracellular
-
 Escherichia coli 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.136 Mg2+ essential Escherichia coli
2.7.1.136 additional information potassium chloride enhances reactivity, it is added in the reaction mixture at 0.06 M Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.136 34000
-
 x * 34000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.136 Escherichia coli Q47396 macrolide 2'-phosphotransferase type I, high levels of activity with 14-member ring macrolides and extremely low levels with 16-member ring macrolides, accession code corresponds to nucleotide sequence for several proteins
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.136 -20°C, Tris-HCl buffer pH 7, 0.06 M KCl, 0.01 M MgCl2, 0.006 M 2-mercaptoethanol, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.136 ATP + erythromycin 87% activity with respect to oleandomycin Escherichia coli ADP + erythromycin-2'-O-phosphate
-
 ?
2.7.1.136 ATP + josamycin 1% activity with respect to oleandomycin Escherichia coli ?
-
 ?
2.7.1.136 ATP + midecamycin 3% activity with respect to oleandomycin Escherichia coli ?
-
 ?
2.7.1.136 ATP + oleandomycin
-
 Escherichia coli ADP + oleandomycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + spectinomycin 5% activity with respect to oleandomycin Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.136 ? x * 34000, SDS-PAGE Escherichia coli