Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wilson, M.P.; Majerus, P.W.
    Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning, and expression of the recombinant enzyme (1996), J. Biol. Chem., 271, 11904-11910.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.134 expresssion in Escherichia coli Homo sapiens
2.7.1.159 DNA and N-terminal amino acid sequence determination and analysis Bos taurus
2.7.1.159 DNA and N-terminal amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.159 0.00008
-
 1D-myo-inositol-1,3,4-trisphosphate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.159 Mg2+
-
 Bos taurus
2.7.1.159 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.134 46000
-
 x * 46000, deduced from nucleotide sequence Homo sapiens
2.7.1.159 45600
-
 x * 45600, about, sequence calculation Homo sapiens
2.7.1.159 48000
-
 x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.134 Bos taurus
-
-
-
2.7.1.134 Homo sapiens Q13572
-
-
2.7.1.159 Bos taurus
-
 calf
-
2.7.1.159 Homo sapiens Q13572
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.134 heparin-agarose, affinity elution with inositol hexakisphosphate, Mono Q Bos taurus
2.7.1.159 12900fold from brain by ion exchange chromatography, and heparin/inositol hexakisphosphate affinity chromatography Bos taurus
2.7.1.159 recombinant His-tagged enzyme from Escherichia coli by adsorption and calmodulin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.134 brain
-
 Homo sapiens
-
2.7.1.134 brain 0.13% of soluble protein Bos taurus
-
2.7.1.134 heart 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 kidney 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 liver 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 liver 20fold lower amounts than in brain Bos taurus
-
2.7.1.134 lung 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 pancreas 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 placenta 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 skeletal muscle 3.6 kilobases transcript detected by Northern blot Homo sapiens
-
2.7.1.134 skeletal muscle 200fold lower amounts than in brain Bos taurus
-
2.7.1.159 brain
-
 Bos taurus
-
2.7.1.159 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.159 25 48 calf brain Bos taurus
2.7.1.159 372
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.134 ATP + myo-inositol-1,3,4-trisphosphate
-
 Bos taurus ADP + myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.134 ATP + myo-inositol-1,3,4-trisphosphate enzyme has both inositol-1,3,4-trisphosphate 5-kinase and inositol-1,3,4-trisphosphate 6-kinase activity Homo sapiens ADP + myo-inositol-1,3,4,6-tetrakisphosphate 5:1 ratio of inositol-tetrakis-1,3,4,6-phosphate and inositol-1,2,4,5-tetrakisphosphate ?
2.7.1.134 ATP + myo-inositol-1,3,4-trisphosphate
-
 Bos taurus ADP + myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.134 ATP + myo-inositol-1,3,4-trisphosphate
-
 Homo sapiens ADP + myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Bos taurus ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Homo sapiens ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Bos taurus ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Homo sapiens ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.134 ? x * 46000, deduced from nucleotide sequence Homo sapiens
2.7.1.159 ? x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE Bos taurus
2.7.1.159 ? x * 45600, about, sequence calculation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.134 inositol 1,3,4-trisphosphate 5/6 kinase
-
 Bos taurus
2.7.1.134 inositol 1,3,4-trisphosphate 5/6 kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.159 37
-
 assay at Bos taurus
2.7.1.159 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.159 7.5
-
 assay at Bos taurus
2.7.1.159 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Bos taurus
2.7.1.159 ATP
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.134 Homo sapiens deduced from amino acid sequence
-
 6.1
2.7.1.159 Homo sapiens sequence calculation
-
 6