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Literature summary extracted from

  • deBelle, I.; Mak, A.S.
    Isolation and characterization of tropomyosin kinase from chicken embryo (1987), Biochim. Biophys. Acta, 925, 17-26.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.28 DTT requirement Gallus gallus

General Stability

EC Number General Stability Organism
2.7.11.28 highly purified enzyme is very unstable upon further purification Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.28 additional information no inhibition by heparin Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.28 0.05
-
 alpha-tropomyosin 37°C, pH 7.5, skeletal alpha-tropomyosin Gallus gallus
2.7.11.28 0.2
-
 ATP 37°C, pH 7.5 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.28 Mg2+ requirement Gallus gallus
2.7.11.28 additional information no activation by Ca2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.28 252000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.28 Gallus gallus
-
-
-
2.7.11.28 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.28 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.28 embryo
-
 Gallus gallus
-
2.7.11.28 additional information not in adult chicken or adult rabbit skeletal muscle Gallus gallus
-
2.7.11.28 additional information not in adult chicken or adult rabbit skeletal muscle Oryctolagus cuniculus
-
2.7.11.28 muscle thigh and leg Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.28 0.0594
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.7.11.28 4°C, in 10% sucrose at least 2 weeks Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.28 ATP + casein
-
 Gallus gallus ADP + phosphocasein
-
 ?
2.7.11.28 ATP + histone II-A
-
 Gallus gallus ADP + phosphohistone II-A
-
 ?
2.7.11.28 ATP + phosphorylase b
-
 Gallus gallus ADP + phosphophosphorylase b
-
 ?
2.7.11.28 ATP + phosvitin
-
 Gallus gallus ADP + phosphophosvitin
-
 ?
2.7.11.28 ATP + tropomyosin beta-tropomyosin from turkey gizzard Gallus gallus ADP + O-phosphotropomyosin
-
 ?
2.7.11.28 ATP + tropomyosin alpha-tropomyosin subunit preferred over beta-tropomyosin subunit Gallus gallus ADP + O-phosphotropomyosin
-
 ?
2.7.11.28 ATP + troponin complex from rabbit skeletal muscle Gallus gallus ADP + phosphotroponin complex
-
 ?
2.7.11.28 additional information no substrates are smooth muscle myosin light chain from turkey gizzard, tropomyosin from platelet and erythrocyte from chicken gizzard and smooth muscle Gallus gallus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.28 37
-
 about Gallus gallus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.11.28 22 40 about 10% of maximal activity at 22°C and about 60% of maximal activity at 40°C Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.28 7.5
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.28 6.2 9.5 about half-maximal activity at pH 6.2 and 9.5 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.28 additional information no activation by cAMP or calmodulin Gallus gallus