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Literature summary extracted from

  • Frampton, E.W.; Wood, W.A.
    Purification and properties of 2-ketogluconokinase from Aerobacter aerogenes (1961), J. Biol. Chem., 236, 2578-2580.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.13 EDTA stimulates Klebsiella aerogenes
2.7.1.13 glutathione stimulates Klebsiella aerogenes

General Stability

EC Number General Stability Organism
2.7.1.13 activity is lost upon dialysis Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.13 4.6
-
 2-dehydro-D-gluconate pH 7.5, 37°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.13 Mg2+ absolutely required for activity Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.13 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.13
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.13 8.083
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.13 -14°C, 14 months, 40% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.13 ATP + 2-dehydro-D-gluconate i.e. 2-dehydro-D-gluconate Klebsiella aerogenes ADP + 2-dehydro-6-phosphogluconate i.e. 6-phospho-2-dehydro-D-gluconate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.13 37
-
 assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.13 7.9 8
-
 Klebsiella aerogenes