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Literature summary extracted from

  • Côté, G.P.; Collins, J.H.; Korn, E.D.
    Identification of three phosphorylation sites on each heavy chain of Acanthamoeba myosin II (1981), J. Biol. Chem., 256, 12811-12816.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 Ca2+/calmodulin weak Acanthamoeba castellanii
2.7.11.7 KCl 60% inhibition at 0.1 M Acanthamoeba castellanii
2.7.11.7 additional information no inhibition by cAMP or cGMP Acanthamoeba castellanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytosol
-
 Acanthamoeba castellanii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ not required Acanthamoeba castellanii
2.7.11.7 Mg2+ requirement Acanthamoeba castellanii
2.7.11.7 Mg2+ cannot be substituted by Ca2+ or Mn2+ Acanthamoeba castellanii
2.7.11.7 additional information no activation by Mn2+ Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin II heavy chain Acanthamoeba castellanii
-
 ADP + myosin II heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Acanthamoeba castellanii
-
 myosin II heavy chain kinase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 partial Acanthamoeba castellanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture
-
 Acanthamoeba castellanii
-

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 4°C, several months Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin II heavy chain
-
 Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain myosin light chains are no substrates Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain incorporation of 3 mol phosphate per mol of myosin II heavy chain, phosphorylation sites are mainly identical in vitro and in vivo Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain phosphorylates serine residues Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 additional information the Mg2+-ATPase activity of the substrate myosin II is inhibited in vitro by its phosphorylation Acanthamoeba castellanii ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 30
-
 assay at Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7 7.5
-
 Acanthamoeba castellanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Acanthamoeba castellanii