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Literature summary extracted from

  • Lynch, T.J.; Brzeska, H.; Baines, I.C.; Korn, E.D.
    Purification of myosin I and myosin I heavy chain kinase from Acanthamoeba castellanii (1991), Methods Enzymol., 196, 12-23.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information enzyme is stimulated by its autophosphorylation Acanthamoeba castellanii
2.7.11.7 phosphatidylserine stimulates the enzymes autophosphorylation activity Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.05
-
 GRGRSSVYS pH 7.5, 30°C Acanthamoeba castellanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 97000
-
 1 * 97000, unphosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii
2.7.11.7 107000
-
 1 * 107000, phosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Acanthamoeba castellanii
-
 ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Acanthamoeba castellanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 affinity chromatography using histone-resin Acanthamoeba castellanii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.7 ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate activation of the enzyme by autophosphorylation is necessary for full catalytic activity Acanthamoeba castellanii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture
-
 Acanthamoeba castellanii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 0.04
-
-
 Acanthamoeba castellanii

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 -20°C, purified, stable up to 1 year Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + GRGRSSVYS synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase enzyme incorporates 6-8 mol phosphate per mol of enzyme Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 additional information the Mg2+-ATPase activity of the substrate myosin I is increased by its phosphorylation and the binding of F-actin Acanthamoeba castellanii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 monomer 1 * 97000, unphosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii
2.7.11.7 monomer 1 * 107000, phosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 30
-
 assay at Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7.5
-
 assay at Acanthamoeba castellanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Acanthamoeba castellanii