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Literature summary extracted from

  • Medley, Q.W.; Bagshaw, W.L.; Truong, T.; Cote, G.P.
    Dictyostelium myosin II heavy-chain kinase A is activated by heparin, DNA and acidic phospholipids and inhibited by polylysine, polyarginine and histones (1992), Biochim. Biophys. Acta, 1175, 7-12.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 DNA autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner Dictyostelium discoideum
2.7.11.7 heparin autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner Dictyostelium discoideum
2.7.11.7 additional information
-
 Dictyostelium discoideum
2.7.11.7 Phospholipid vesicles autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner Dictyostelium discoideum
2.7.11.7 Phospholipid vesicles composed of phosphatidylserine or phosphatidylinositol, not phosphatidylcholine Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 histone
-
 Dictyostelium discoideum
2.7.11.7 Positively charged polypeptides strong inhibition, e.g. poly-(D-Lys), poly-(L-Lys), poly-(L-Arg) of different molecular weights Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytosol
-
 Dictyostelium discoideum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ not required Dictyostelium discoideum
2.7.11.7 Mg2+ requirement Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum involved in regulation of myosin II filament assembly ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum
-
 two heavy chain kinases: one in growth-phase cells and one in starved, developing cells
-
2.7.11.7 Dictyostelium discoideum
-
 gene MHCK A
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture growth-phase cells contain enzyme form MHCK A Dictyostelium discoideum
-
2.7.11.7 cell culture starved-developing cells contain enzyme form MHCK B Dictyostelium discoideum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylation of residues Thr1833 and Thr2029 Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain involved in regulation of myosin II filament assembly Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + synthetic peptides peptide MH-3, corresponding to myosin II heavy chain from residues 2020 to 2035 except that Ser-2026 and Thr-2031 have been replaced by alanine Dictyostelium discoideum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
 assay at Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7.5
-
 assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.7 additional information
-
 additional information inhibition by positively charged polypeptides Dictyostelium discoideum