Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Communi, D.; Vanweyenberg, V.; Erneux, C.
    Purification and biochemical properties of a high-molecular-mass inositol 1,4,5-trisphosphate 3-kinase isoenzyme in human platelets (1994), Biochem. J., 298, 669-673.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.127 calpain inhibitors stabilize during purification Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.002
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.4, 37°C, platelet enzyme Homo sapiens
2.7.1.127 2.5
-
 ATP pH 7.4, 37°C, platelet enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 soluble
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Ca2+ 17fold stimulation by Ca2+/calmodulin at saturating calmodulin and 0.01 mM free Ca2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Homo sapiens
-
 3 isoenzymes: A and B, brain, and high-molecular weight platelet isoenzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 4662fold Homo sapiens
2.7.1.127 calmodulin-Sepharose affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 platelet
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 0.75 1.45 pH 7.4, 37°C, platelet, in presence of free Ca2+ and calmodulin Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 monomer 1 * 69000-70000, platelet, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Homo sapiens
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Calmodulin 17fold stimulation by Ca2+/calmodulin at saturating calmodulin and 0.01 mM free Ca2+ Homo sapiens