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Literature summary extracted from

  • Scott-Woo, G.C.; Walsh, M.P.
    Autophosphorylation of smooth-muscle caldesmon (1988), Biochem. J., 252, 463-472.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ requirement Gallus gallus
2.7.11.17 Ca2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 141000
-
 x * 141000, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.17 ATP + caldesmon Bos taurus
-
 ADP + caldesmon phosphate
-
 ?
2.7.11.17 ATP + caldesmon Gallus gallus caldesmon plays a role in the regulation of smooth muscle contraction ADP + caldesmon phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Bos taurus
-
-
-
2.7.11.17 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17
-
 Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.17 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate the reaction is an intramolecular autophosphorylation which is site-specific: predominantly serine with some threonine and no tyrosine residues are phosphorylated Gallus gallus
a
2.7.11.17 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate the reaction is an intramolecular autophosphorylation which is site-specific: predominantly serine with some threonine and no tyrosine residues are phosphorylated Bos taurus
a
2.7.11.17 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate enzyme appears to be identical with caldesmon Gallus gallus
a
2.7.11.17 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate enzyme appears to be identical with caldesmon Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 aorta
-
 Bos taurus
-
2.7.11.17 gizzard smooth muscle Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.00027
-
 histone III-S Gallus gallus
2.7.11.17 0.00054
-
 aortic caldesmon Gallus gallus
2.7.11.17 0.00063
-
 synapsin Gallus gallus
2.7.11.17 0.00073
-
 autophosphorylation Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + caldesmon
-
 Gallus gallus ADP + caldesmon phosphate
-
 r
2.7.11.17 ATP + caldesmon
-
 Bos taurus ADP + caldesmon phosphate
-
 ?
2.7.11.17 ATP + caldesmon caldesmon plays a role in the regulation of smooth muscle contraction Gallus gallus ADP + caldesmon phosphate
-
 ?
2.7.11.17 ATP + casein
-
 Gallus gallus ADP + phosphocasein
-
 ?
2.7.11.17 ATP + histone histone V-S Bos taurus ADP + phosphohistone
-
 ?
2.7.11.17 ATP + histone calf thymus histone II-S, poor substrate Gallus gallus ADP + phosphohistone
-
 ?
2.7.11.17 ATP + histone histone III-S Gallus gallus ADP + phosphohistone
-
 ?
2.7.11.17 ATP + histone histone III-S Bos taurus ADP + phosphohistone
-
 ?
2.7.11.17 ATP + myosin
-
 Bos taurus ADP + phosphomyosin
-
 ?
2.7.11.17 ATP + myosin isolated light chain of smooth-muscle myosin, phosphorylated at 80% the rate of caldesmon Gallus gallus ADP + phosphomyosin
-
 ?
2.7.11.17 ATP + synapsin
-
 Bos taurus ADP + phosphosynapsin
-
 ?
2.7.11.17 ATP + synapsin brain synapsin best substrate of chicken gizzard caldesmon kinase Gallus gallus ADP + phosphosynapsin
-
 ?
2.7.11.17 additional information no substrates are bovine cardiac C-protein, bovine brain fodrin, rabbit skeletal muscle glycogen synthase, phosphorylase B, troponon (I + T + C), actin, tropomyosin, smooth muscle actin, filamin, vinculin, alpha-actinin, protamine or phosvitin Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.17 ? x * 141000, SDS-PAGE Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 Calmodulin requirement Gallus gallus
2.7.11.17 Calmodulin requirement Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.17 Gallus gallus isoelectric focusing, multiple charge-variants 5.9 5.78
2.7.11.17 Bos taurus isoelectric focusing, multiple charge-variants 5.9 5.78