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Literature summary extracted from

  • Izu, H.; Adachi, O.; Yamada, M.
    Purification and characterization of the Escherichia coli thermoresistant glucokinase encoded by the gntK gene (1996), FEBS Lett., 394, 14-16.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.12 0.02
-
 D-gluconate
-
 Escherichia coli
2.7.1.12 0.042
-
 D-gluconate 25°C, pH 7 Escherichia coli
2.7.1.12 0.045
-
 ATP
-
 Escherichia coli
2.7.1.12 0.123
-
 ATP 25°C, pH 7 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.12 18400
-
 2 * 18400, SDS-PAGE Escherichia coli
2.7.1.12 29500
-
 alpha3, 3 * 29500 Escherichia coli
2.7.1.12 38000
-
 gel filtration Escherichia coli
2.7.1.12 100000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.12 ATP + D-gluconate Escherichia coli
-
 ADP + 6-phospho-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.12 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.12
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.12 30
-
-
 Escherichia coli
2.7.1.12 250
-
 thermoresistant gluconokinase encoded by th gntK Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.12 ATP + D-gluconate
-
 Escherichia coli ADP + 6-phospho-D-gluconate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.12 homodimer 2 * 18400, SDS-PAGE Escherichia coli
2.7.1.12 trimer alpha3, 3 * 29500 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.12 45
-
 thermoresistant gluconokinase encoded by th gntK Escherichia coli
2.7.1.12 55
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.12 50
-
 after 40 min, 50% loss of activity Escherichia coli