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Literature summary extracted from

  • Higashi, K.; Fukunaga, K.; Matsui, K.; Maeyama, M.; Miyamoto, E.
    Purification and characterization of myosin light-chain kinase from porcine myometrium and its phosphorylation and modulation by cyclic AMP-dependent protein kinase (1983), Biochim. Biophys. Acta, 747, 232-240.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 autophosphorylation no activation Sus scrofa
2.7.11.18 additional information no activation by phosphatidylserine Sus scrofa
2.7.11.18 additional information no activation by cAMP Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 cAMP-dependent protein kinase phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.02 0.027 turkey gizzard myosin light chain 30°C, pH 7 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 soluble
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ Km-value: 0.0003 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 130000
-
 x * 130000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Sus scrofa
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 myometrium
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 7.9
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.11.18 -20°C, quite unstable on storage Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are myosin heavy chain and phosvitin Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is protamine Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for regulatory or P-light-chain Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for 18 kDa light chain Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase kinase Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Sus scrofa ADP + myosin light chain phosphate
-
 ?
2.7.11.18 additional information no intramolecular autophosphorylation in the absence of acceptor substrate Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 ? x * 130000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Sus scrofa
2.7.11.18 Calmodulin lower affinity for Ca2+/caldesmon after phosphorylation by cAMP-dependent protein kinase, Ka: 0.0000006 mM Sus scrofa