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Literature summary extracted from

  • Popov, K.M.; Shimomura, Y.; Hawes, J.W.; Harris, R.A.
    Branched-chain alpha-keto acid dehydrogenase kinase (2000), Methods Enzymol., 324, 162-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.4
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.4 2-Chloroisohexanoate 50% inhibition at 0.014 mM Rattus norvegicus
2.7.11.4 Dichloroacetate 50% inhibition at 1.8 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.4 44000 45000 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.4 liver enzyme, heart enzyme and recombinant enzyme expressed in Escherichia coli Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.4 heart
-
 Rattus norvegicus
-
2.7.11.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.4 0.0268
-
 liver enzyme Rattus norvegicus
2.7.11.4 0.0357 0.09 heart enzyme, depending on purification method Rattus norvegicus
2.7.11.4 0.05
-
 recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.4 ATP + histone II-S
-
 Rattus norvegicus ?
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)]
-
 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?