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Literature summary extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Deoxyguanosine kinase from human placenta (1982), Biochim. Biophys. Acta, 709, 265-272.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.113 CTP activation, with ATP as substrate at pH 7 Homo sapiens
2.7.1.113 dTTP 1 mM, 3-4fold activation of deoxyguanosine kinase at pH 7.0 Homo sapiens
2.7.1.113 dUTP activation, with ATP as substrate, at pH 7 Homo sapiens
2.7.1.113 additional information not activated by dTDP Homo sapiens
2.7.1.113 UTP slight inhibition at pH 6 Homo sapiens
2.7.1.113 UTP stimulation with ATP as substrate, at pH 7 Homo sapiens

General Stability

EC Number General Stability Organism
2.7.1.113 complete inactivation after freezing at -20°C or -70°C Homo sapiens
2.7.1.113 loss of activity in dilute solution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.113 ADP 1 mM, 45% inhibition at pH 6.0 Homo sapiens
2.7.1.113 arabinoadenosine
-
Homo sapiens
2.7.1.113 arabinocytidine
-
Homo sapiens
2.7.1.113 CTP
-
Homo sapiens
2.7.1.113 CuSO4
-
Homo sapiens
2.7.1.113 dADP 1 mM, 49% inhibition at pH 6.0 Homo sapiens
2.7.1.113 dATP 1 mM, 71% inhibition at pH 6.0 Homo sapiens
2.7.1.113 dCDP
-
Homo sapiens
2.7.1.113 dCTP
-
Homo sapiens
2.7.1.113 deoxyadenosine
-
Homo sapiens
2.7.1.113 deoxyinosine
-
Homo sapiens
2.7.1.113 dGDP 0.0021 mM, 50% inhibition, reversed by 0.2 mM dTTP Homo sapiens
2.7.1.113 dGMP 1 mM, 64% inhibition at pH 6.0 Homo sapiens
2.7.1.113 dGTP 0.001 mM, 50% inhibition, 1 mM, complete inhibition, reversed by 0.2 mM dTTP Homo sapiens
2.7.1.113 dTDP 1 mM, 51% inhibition at pH 6.0 Homo sapiens
2.7.1.113 dUTP
-
Homo sapiens
2.7.1.113 EDTA
-
Homo sapiens
2.7.1.113 GDP 1 mM, 50% inhibition at pH 6.0 Homo sapiens
2.7.1.113 GTP
-
Homo sapiens
2.7.1.113 guanosine
-
Homo sapiens
2.7.1.113 IDP 1 mM, 58% inhibition at pH 6.0 Homo sapiens
2.7.1.113 additional information not inhibited by deoxycytidine, adenosine; not inhibited by dTTP; not inhibited by inosine, cytidine, uridine, deoxythymidine, deoxyuridine Homo sapiens
2.7.1.113 ribavirin
-
Homo sapiens
2.7.1.113 UTP
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.113 0.0025
-
deoxyguanosine pH 6.0, 37°C Homo sapiens
2.7.1.113 0.13
-
ATP pH 6.0, 37°C Homo sapiens
2.7.1.113 0.13
-
ATP ATP in form of MgATP2- Homo sapiens
2.7.1.113 0.63
-
deoxyadenosine pH 6.0, 37°C Homo sapiens
2.7.1.113 2.2
-
dTTP pH 6.0, 37°C Homo sapiens
2.7.1.113 2.2
-
dTTP dTTP in form of MgdTTP2- Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.113 Ba2+ slight activation Homo sapiens
2.7.1.113 Ca2+ activation Homo sapiens
2.7.1.113 Ca2+ 60% of activity with Mg2+ Homo sapiens
2.7.1.113 Co2+ 59% of activity with Mg2+ Homo sapiens
2.7.1.113 Cr2+ slight activation Homo sapiens
2.7.1.113 Fe2+ 33% of activity with Mg2+ Homo sapiens
2.7.1.113 Mg2+ required for activity Homo sapiens
2.7.1.113 Mn2+ activation, as effective as Mg2+ Homo sapiens
2.7.1.113 additional information not activated by Cu2+ Homo sapiens
2.7.1.113 Ni2+ slight activation Homo sapiens
2.7.1.113 Zn2+ 45% of activity with Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.113 29000
-
2 * 29000, second mayor band at 35000 Da, SDS-PAGE Homo sapiens
2.7.1.113 58000
-
gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.113 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.113 ammonium sulfate, affinity chromatography on AMP-Sepharose and Blue-Sepharose, DEAE-cellulose, Sephadex G-75 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.113 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.113 0.0103
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.113 4°C, 1 week, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.113 ATP + deoxyadenosine 4.8%, 25.8% and 93.5% of activity with deoxyguanosine at 0.01 mM, 0.1 mM and 1 mM deoxyguanosine, respectively Homo sapiens ADP + dAMP
-
?
2.7.1.113 ATP + deoxyguanosine no activity with deoxycytidine Homo sapiens ADP + dGMP
-
?
2.7.1.113 ATP + deoxyguanosine ATP can be replaced by dUTP Homo sapiens ADP + dGMP
-
?
2.7.1.113 ATP + deoxyguanosine no activity with dATP and GTP Homo sapiens ADP + dGMP
-
?
2.7.1.113 ATP + deoxyguanosine no activity with dGTP Homo sapiens ADP + dGMP
-
?
2.7.1.113 ATP + deoxyguanosine ATP can be replaced by dTTP, CTP, dCTP and UTP Homo sapiens ADP + dGMP
-
?
2.7.1.113 CTP + deoxyguanosine 62% of activity with ATP at pH 6.0, 1.8fold higher activity than with ATP at pH 7.0 Homo sapiens CDP + dGMP
-
?
2.7.1.113 dCTP + deoxyguanosine
-
Homo sapiens dCDP + dGMP
-
?
2.7.1.113 dTTP + deoxyguanosine 83% of activity with ATP at pH 6.0, 2.8fold higher activity than with ATP at pH 7.0 Homo sapiens dTDP + dGMP
-
?
2.7.1.113 dTTP + deoxyguanosine
-
Homo sapiens ?
-
?
2.7.1.113 dUTP + deoxyguanosine 69% of activity with ATP at pH 6.0, 1.7fold higher activity than with ATP at pH 7.0 Homo sapiens dUDP + dGMP
-
?
2.7.1.113 UTP + deoxyguanosine
-
Homo sapiens UDP + dGMP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.113 dimer 2 * 29000, second mayor band at 35000 Da, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.113 6
-
cosubstrate ATP Homo sapiens
2.7.1.113 6.8
-
cosubstrate ATP, addition of dTTP Homo sapiens
2.7.1.113 7
-
cosubstrate dTTP Homo sapiens