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Literature summary extracted from

  • Fordyce, A.M.; Moore, C.H.; Pritchard, G.G.
    Phosphofructokinase from Streptococcus lactis (1982), Methods Enzymol., 90, 77-82.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Lactococcus lactis
2.7.1.11 NH4+
-
 Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.11 4 mg/ml PFK in 25 mM sodium phosphate buffer pH 7.0, is dialyzed at 4°C against 25 mM phosphate buffer, pH 7.0, containing 10 mM 2-mercaptoethanol, 1 mM MgSO4, 1 mM EDTA, 2 mM ATP, 2 mM FDP and 23% ammonium sulfate, crystals appear after 8-10 weeks Lactococcus lactis

General Stability

EC Number General Stability Organism
2.7.1.11 dilution inactivates Lactococcus lactis
2.7.1.11 fructose 6-phosphate, ATP, ADP, citrate, KCl or ammonium sulfate does not stabilize Lactococcus lactis
2.7.1.11 glycerol prevents inactivation of dilute cytosolic enzyme solution Lactococcus lactis
2.7.1.11 glycerol stabilizes Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 AMP 5 mM, 80% inhibition Lactococcus lactis
2.7.1.11 D-fructose 1,6-bisphosphate 5 mM, 25% inhibition Lactococcus lactis
2.7.1.11 Li+ weak Lactococcus lactis
2.7.1.11 Na+ weak Lactococcus lactis
2.7.1.11 phosphate 10 mM, 50% inhibition Lactococcus lactis
2.7.1.11 phosphoenolpyruvate 5 mM; 64% inhibition Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.18
-
 ATP pH 7.5 Lactococcus lactis
2.7.1.11 0.18
-
 ATP ATP in form of MgATP2- Lactococcus lactis
2.7.1.11 0.25
-
 D-fructose 6-phosphate pH 7.5 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Co2+ 50% of activity with Mg2+- Lactococcus lactis
2.7.1.11 K+ slight activation at low concentrations Lactococcus lactis
2.7.1.11 Mg2+ required for activity Lactococcus lactis
2.7.1.11 Mg2+ maximal activity at 2-5 mM Lactococcus lactis
2.7.1.11 Mn2+ activation Lactococcus lactis
2.7.1.11 Mn2+ 75% of activity with Mg2+ Lactococcus lactis
2.7.1.11 Ni2+ activation, 12% of activity with Lactococcus lactis
2.7.1.11 Zn2+ activation Lactococcus lactis
2.7.1.11 Zn2+ 35% of activity with Mg2+ Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 144500
-
 gel filtration Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Lactococcus lactis
-
-
-
2.7.1.11 Lactococcus lactis C10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 161
-
-
 Lactococcus lactis

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -12°C, Tris-glycerol buffer, pH 7.5, 50% v/v glycerol, up to 6 months, 10-20% loss of activity Lactococcus lactis
2.7.1.11 4°C, less stable at pH 6 and 8 than at neutral pH-values Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Lactococcus lactis ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Lactococcus lactis C10 ADP + D-fructose 1,6-bisphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.2 7.8
-
 Lactococcus lactis