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Literature summary extracted from

  • Hofmann, E.; Kopperschläger, G.
    Phosphofructokinase from yeast (1982), Methods Enzymol., 90, 49-60.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Saccharomyces cerevisiae
2.7.1.11 ADP slight Saccharomyces cerevisiae
2.7.1.11 D-fructose 2,6-bisphosphate strong positive allosteric action on PFK Saccharomyces cerevisiae
2.7.1.11 NH4+ increases maximum activity of PFK and the affinity of PFK to fructose 6-phosphate Saccharomyces cerevisiae
2.7.1.11 phosphate synergistic with AMP Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.7.1.11 dilution inactivates Saccharomyces cerevisiae
2.7.1.11 dilution leads to enzyme dissociation Saccharomyces cerevisiae
2.7.1.11 PMSF stabilizes during purification Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP
-
 Saccharomyces cerevisiae
2.7.1.11 citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Saccharomyces cerevisiae
2.7.1.11 MgATP2-
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information pH-dependence of kinetic properties of cytosolic and plastid isozymes Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 K+
-
 Saccharomyces cerevisiae
2.7.1.11 Mg2+ required for activity Saccharomyces cerevisiae
2.7.1.11 Mg2+ MgATP is the active substrate Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 112000
-
 alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae
2.7.1.11 118000
-
 alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae
2.7.1.11 835000
-
 sedimentation equilibrium analysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate Saccharomyces cerevisiae dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Saccharomyces cerevisiae
-
 baker's yeast, preferred source: pitching yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 ammonium sulfate, Cibacron Blue F3G-A, gel filtration, ion-exchange Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.11 ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate mechanism Saccharomyces cerevisiae
a
2.7.1.11 ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate structure-oriented allosteric four-state model describing substrate and effector actions on PFK Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 60
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 0°C, dilute enzyme solution in 5 mM imidazole-HCl buffer, pH 7, 1 mM D-fructose 6-phosphate, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 6 h, 20% loss of activity Saccharomyces cerevisiae
2.7.1.11 4°C, concentrated enzyme solution, 50 mM potassium phosphate buffer, pH 7, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 1 mM fructose 6-phosphate, several months Saccharomyces cerevisiae
2.7.1.11 room temperature, 20% loss of activity within 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 octamer alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 additional information
-
 pH-dependence of kinetic properties Saccharomyces cerevisiae
2.7.1.11 7
-
 optimum depends on the ATP and fructose 6-phosphate concentration Saccharomyces cerevisiae