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Literature summary extracted from

  • Kamemoto, E.S.; Mansour, T.E.
    Phosphofructokinase in the liver fluke Fasciola hepatica. Purification and kinetic changes by phosphorylation (1986), J. Biol. Chem., 261, 4346-4351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Fasciola hepatica
2.7.1.11 cAMP activation Fasciola hepatica
2.7.1.11 cAMP-dependent protein kinase activation by phosphorylation Fasciola hepatica
2.7.1.11 NH4+
-
 Fasciola hepatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP 1 mM, almost complete inhibition of native PFK, phosphorylated PFK is less sensitive to inhibition Fasciola hepatica
2.7.1.11 ATP
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information kinetic study Ovis aries
2.7.1.11 additional information
-
 additional information kinetic study Fasciola hepatica
2.7.1.11 additional information
-
 additional information kinetic properties of phosphorylated enzyme Ovis aries
2.7.1.11 additional information
-
 additional information kinetic properties of phosphorylated enzyme Fasciola hepatica
2.7.1.11 1.8
-
 D-fructose 6-phosphate pH 8.0, phosphorylated PFK Fasciola hepatica
2.7.1.11 7
-
 D-fructose 6-phosphate pH 8.0, native PFK Fasciola hepatica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Ovis aries
2.7.1.11 Mg2+ required for activity Fasciola hepatica

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Fasciola hepatica
-
-
-
2.7.1.11 Ovis aries
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.11 side-chain modification in vitro phosphorylation with cAMP-dependent protein kinase, 0.1-0.2 mol phosphate/mol of protomer, phosphorylation increases activity of PFK and reduces sensitivity to ATP inhibition Fasciola hepatica

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 DEAE-cellulose, ammonium sulfate, AMP-agarose Fasciola hepatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 brain
-
 Ovis aries
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 4°C, enhanced stability in ammonium sulfate suspension Fasciola hepatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Ovis aries ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Fasciola hepatica ADP + D-fructose 1,6-bisphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.5
-
 ATP pH 8.0, native PFK Fasciola hepatica
2.7.1.11 1.4
-
 ATP pH 8.0, phosphorylated PFK Fasciola hepatica