BRENDA - Enzyme Database

Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae

Fernandez, F.; Shridas, P.; Jiang, S.; Aebi, M.; Waechter, C.J.; Glycobiology 12, 555-562 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.108
-
Saccharomyces cerevisiae
2.7.1.108
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Homo sapiens
16020
-
2.7.1.108
membrane
-
Saccharomyces cerevisiae
16020
-
2.7.1.108
microsome
-
Saccharomyces cerevisiae
-
-
2.7.1.108
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.108
59000
60000
SDS-PAGE
Homo sapiens
2.7.1.108
59270
-
predicted from cDNA open reading frame
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Homo sapiens
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Homo sapiens
?
2.7.1.108
CTP + dolichol
Saccharomyces cerevisiae
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Homo sapiens
-
clone KIAA1094 from a human brain library
-
2.7.1.108
Saccharomyces cerevisiae
P20048
yeast, SEC59 gene
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640401
Homo sapiens
CDP + dolichyl phosphate
-
-
-
?
2.7.1.108
CTP + dolichol
-
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
-
-
?
2.7.1.108
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Homo sapiens
CDP + dolichyl phosphate
-
640401
Homo sapiens
?
2.7.1.108
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
640401
Saccharomyces cerevisiae
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.108
-
Saccharomyces cerevisiae
2.7.1.108
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Homo sapiens
16020
-
2.7.1.108
membrane
-
Saccharomyces cerevisiae
16020
-
2.7.1.108
microsome
-
Homo sapiens
-
-
2.7.1.108
microsome
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.108
59000
60000
SDS-PAGE
Homo sapiens
2.7.1.108
59270
-
predicted from cDNA open reading frame
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Homo sapiens
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Homo sapiens
?
2.7.1.108
CTP + dolichol
Saccharomyces cerevisiae
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640401
Homo sapiens
CDP + dolichyl phosphate
-
-
-
?
2.7.1.108
CTP + dolichol
-
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
-
-
?
2.7.1.108
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Homo sapiens
CDP + dolichyl phosphate
-
640401
Homo sapiens
?
2.7.1.108
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
640401
Saccharomyces cerevisiae
?