BRENDA - Enzyme Database

Properties of brain dolichol kinase activity solubilized with a zwitterionic detergent

Sumbilla, C.; Waechter, C.J.; Arch. Biochem. Biophys. 238, 75-82 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
(R)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
2.7.1.108
(S)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
2.7.1.108
phosphatidylcholine
stimulated by after dialysis
Bos taurus
2.7.1.108
phosphatidylethanolamine
stimulated by after dialysis
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Bos taurus
2.7.1.108
dCDP
-
Bos taurus
2.7.1.108
EDTA
-
Bos taurus
2.7.1.108
additional information
not inhibited by CMP, ADP, GDP or UDP
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.0077
-
CTP
pH 7.2, 37°C
Bos taurus
2.7.1.108
0.0091
-
dCTP
pH 7.2, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Zn2+
markedly stimulated by
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Bos taurus
-
calf
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.108
-17°C, 65% of original activity retains when stored for 6 weeks
Bos taurus
2.7.1.108
4°C, no detectable loss of activity when solubilized extract is kept for 24 h
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640396
Bos taurus
CDP + dolichyl phosphate
-
640396
Bos taurus
?
2.7.1.108
dCTP + dolichol
-
640396
Bos taurus
dCDP + dolichyl phosphate
-
640396
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7.5
-
-
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.108
0.121
-
CDP
pH 7.2, 37°C
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
(R)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
2.7.1.108
(S)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
2.7.1.108
phosphatidylcholine
stimulated by after dialysis
Bos taurus
2.7.1.108
phosphatidylethanolamine
stimulated by after dialysis
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Bos taurus
2.7.1.108
dCDP
-
Bos taurus
2.7.1.108
EDTA
-
Bos taurus
2.7.1.108
additional information
not inhibited by CMP, ADP, GDP or UDP
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.108
0.121
-
CDP
pH 7.2, 37°C
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.0077
-
CTP
pH 7.2, 37°C
Bos taurus
2.7.1.108
0.0091
-
dCTP
pH 7.2, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Zn2+
markedly stimulated by
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.108
-17°C, 65% of original activity retains when stored for 6 weeks
Bos taurus
2.7.1.108
4°C, no detectable loss of activity when solubilized extract is kept for 24 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640396
Bos taurus
CDP + dolichyl phosphate
-
640396
Bos taurus
?
2.7.1.108
dCTP + dolichol
-
640396
Bos taurus
dCDP + dolichyl phosphate
-
640396
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7.5
-
-
Bos taurus