BRENDA - Enzyme Database

Calmodulin-dependent enzymic phosphorylation of dolichol in Tetrahymena pyriformis

Gandhi, C.R.; Keenan, R.W.; Biochem. J. 216, 317-323 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
Triton X-100
-
Tetrahymena pyriformis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
chlorpromazine
-
Tetrahymena pyriformis
2.7.1.108
Trifluoperazine
-
Tetrahymena pyriformis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.07
-
dolichol
pH 7.4, 37C
Tetrahymena pyriformis
2.7.1.108
3
-
CTP
pH 7.4, 37C
Tetrahymena pyriformis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
required for maxiumum activity
Tetrahymena pyriformis
2.7.1.108
Mg2+
required for maxiumum activity
Tetrahymena pyriformis
2.7.1.108
Mn2+
activated by
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Tetrahymena pyriformis
-
CDP + dolichyl phosphate
-
Tetrahymena pyriformis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Tetrahymena pyriformis
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.108
-20C, stable for at least 15 days
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640394
Tetrahymena pyriformis
CDP + dolichyl phosphate
-
640394
Tetrahymena pyriformis
?
2.7.1.108
additional information
ATP, UTP and GTP are ineffective
640394
Tetrahymena pyriformis
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7.4
-
Tris/maleate buffer, second optimum at pH 9.0
Tetrahymena pyriformis
2.7.1.108
9
-
glycine buffer, second optimum at pH 7.4
Tetrahymena pyriformis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
6.6
9.6
2 optima, about half-maximal activity at pH 6.6, pH 8.5 and pH 9.6
Tetrahymena pyriformis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
Triton X-100
-
Tetrahymena pyriformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
chlorpromazine
-
Tetrahymena pyriformis
2.7.1.108
Trifluoperazine
-
Tetrahymena pyriformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.07
-
dolichol
pH 7.4, 37C
Tetrahymena pyriformis
2.7.1.108
3
-
CTP
pH 7.4, 37C
Tetrahymena pyriformis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
required for maxiumum activity
Tetrahymena pyriformis
2.7.1.108
Mg2+
required for maxiumum activity
Tetrahymena pyriformis
2.7.1.108
Mn2+
activated by
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Tetrahymena pyriformis
-
CDP + dolichyl phosphate
-
Tetrahymena pyriformis
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.108
-20C, stable for at least 15 days
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640394
Tetrahymena pyriformis
CDP + dolichyl phosphate
-
640394
Tetrahymena pyriformis
?
2.7.1.108
additional information
ATP, UTP and GTP are ineffective
640394
Tetrahymena pyriformis
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7.4
-
Tris/maleate buffer, second optimum at pH 9.0
Tetrahymena pyriformis
2.7.1.108
9
-
glycine buffer, second optimum at pH 7.4
Tetrahymena pyriformis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
6.6
9.6
2 optima, about half-maximal activity at pH 6.6, pH 8.5 and pH 9.6
Tetrahymena pyriformis