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Literature summary extracted from

  • Gandhi, C.R.; Keenan, R.W.
    Calmodulin-dependent enzymic phosphorylation of dolichol in Tetrahymena pyriformis (1983), Biochem. J., 216, 317-323.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.108 Triton X-100
-
 Tetrahymena pyriformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.108 chlorpromazine
-
 Tetrahymena pyriformis
2.7.1.108 Trifluoperazine
-
 Tetrahymena pyriformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.108 0.07
-
 dolichol pH 7.4, 37°C Tetrahymena pyriformis
2.7.1.108 3
-
 CTP pH 7.4, 37°C Tetrahymena pyriformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.108 Ca2+ required for maxiumum activity Tetrahymena pyriformis
2.7.1.108 Mg2+ required for maxiumum activity Tetrahymena pyriformis
2.7.1.108 Mn2+ activated by Tetrahymena pyriformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.108 CTP + dolichol Tetrahymena pyriformis
-
 CDP + dolichyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Tetrahymena pyriformis
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.108 -20°C, stable for at least 15 days Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Tetrahymena pyriformis CDP + dolichyl phosphate
-
 ?
2.7.1.108 additional information ATP, UTP and GTP are ineffective Tetrahymena pyriformis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.108 7.4
-
 Tris/maleate buffer, second optimum at pH 9.0 Tetrahymena pyriformis
2.7.1.108 9
-
 glycine buffer, second optimum at pH 7.4 Tetrahymena pyriformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.108 6.6 9.6 2 optima, about half-maximal activity at pH 6.6, pH 8.5 and pH 9.6 Tetrahymena pyriformis