BRENDA - Enzyme Database

Extraction and detergent/lipid activation of dolichol kinase

Allen, C.M.; Muth, J.D.; Gildersleeve, N.; Biochim. Biophys. Acta 712, 33-41 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
dimyristoylphosphatidylcholine
stimulatory in 1:1 ratio with deoxycholate
Bos taurus
2.7.1.108
additional information
sodium dodecylsulfate is ineffective in stimulating enzymic activity
Bos taurus
2.7.1.108
Triton X-100
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
Ca2+
above 6 mM
Bos taurus
2.7.1.108
Co2+
above 6 mM
Bos taurus
2.7.1.108
Mn2+
above 6 mM
Bos taurus
2.7.1.108
additional information
Mg2+ is not inhibitory
Bos taurus
2.7.1.108
Zn2+
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.15
-
CTP
pH 7.5, 35C, in deoxycholate/dimyristoylphosphatidylcholine 1:1
Bos taurus
2.7.1.108
0.55
-
CTP
pH 7.5, 35C, in Triton X-100/dimyristoylphosphatidylcholine 1:1
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Bos taurus
16020
-
2.7.1.108
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Co2+
divalent cation required for activity, Mg2+, Ca2+ and Co2+ are equally effective
Bos taurus
2.7.1.108
Mg2+
enzyme is dependent on divalent cations
Bos taurus
2.7.1.108
additional information
Zn2+ is not effective
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Bos taurus
-
bovine
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.108
partially
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.108
-20C, stored frozen as pellet for months with little loss of activity
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640392
Bos taurus
CDP + dolichyl phosphate
-
640392
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
8.5
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
6.6
10.2
broad pH-activity profile with good activity observed from pH 7.0-10.0, about half-maximal activity at pH 6.6 and 10.2
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
dimyristoylphosphatidylcholine
stimulatory in 1:1 ratio with deoxycholate
Bos taurus
2.7.1.108
additional information
sodium dodecylsulfate is ineffective in stimulating enzymic activity
Bos taurus
2.7.1.108
Triton X-100
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
Ca2+
above 6 mM
Bos taurus
2.7.1.108
Co2+
above 6 mM
Bos taurus
2.7.1.108
Mn2+
above 6 mM
Bos taurus
2.7.1.108
additional information
Mg2+ is not inhibitory
Bos taurus
2.7.1.108
Zn2+
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.15
-
CTP
pH 7.5, 35C, in deoxycholate/dimyristoylphosphatidylcholine 1:1
Bos taurus
2.7.1.108
0.55
-
CTP
pH 7.5, 35C, in Triton X-100/dimyristoylphosphatidylcholine 1:1
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Bos taurus
16020
-
2.7.1.108
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Co2+
divalent cation required for activity, Mg2+, Ca2+ and Co2+ are equally effective
Bos taurus
2.7.1.108
Mg2+
enzyme is dependent on divalent cations
Bos taurus
2.7.1.108
additional information
Zn2+ is not effective
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.108
partially
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.108
-20C, stored frozen as pellet for months with little loss of activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640392
Bos taurus
CDP + dolichyl phosphate
-
640392
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
8.5
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
6.6
10.2
broad pH-activity profile with good activity observed from pH 7.0-10.0, about half-maximal activity at pH 6.6 and 10.2
Bos taurus